10个Y-STR基因座复合扩增体系建立及应用

发布时间：2018-11-29 12:19

【摘要】：目的建立DYF404S1a/b、DYF399S1 a/b/c、DYF403S1 a1-3/b、DYS576、DYS570、DYS627、DYS588、DYS447、DYS446、DYS449共10个Y-STR基因座的四色荧光复合扩增体系,调查河南汉族群体中的遗传多态性,评价其法医学应用价值。方法采集500名河南汉族男性个体血液样本,用6-FAM、HEX和TAMRA荧光染料分组标记10个Y-STR基因座,PCR扩增产物采用ABI3130XL遗传分析仪进行分离,Gene Mapper ID v3.2软件分型。结果采用本文方法,检测的最低基因组DNA量为0.05ng;群体调查在上述10个Y-STR基因座分别检测出8~28个等位基因,GD值在0.472 0~0.999 0范围之间,DP值在0.471 1~0.997 0之间。10个基因座所组成的单体型共观察到497种,其中仅出现1次的单体型有494种,HD值达到0.999 908 75,DC值为0.994 0。结论建立的10个Y-STR基因座荧光复合扩增体系灵敏度较高,在河南汉族群体中具有较高的遗传多态性,可以在相关研究和应用中选择使用。
[Abstract]:Objective to establish a four-color fluorescent multiplex amplification system for 10 Y-STR loci of DYS570, DYS588, DYS446 and DYS449 in Henan Han population, and to investigate the genetic polymorphisms of DYS576DYS576DYS570 and DYS588DYS446DYS449 in Henan Han population, and to investigate the genetic polymorphisms of DYS576DYS570DYS570 and DYS588DYS446DYS449 in Henan Han population. To evaluate the value of forensic application. Methods Blood samples were collected from 500 Han Chinese men in Henan province. Ten Y-STR loci were labeled with 6-FAMHEX and TAMRA fluorescent dyes. The PCR amplification products were classified into, Gene Mapper ID v3.2 by ABI3130XL genetic analyzer. Results using this method, the minimum genomic DNA was 0.05ng. A total of 8 ~ 28 alleles were detected in the 10 Y-STR loci mentioned above. The GD value ranged from 0.472 to 0.999 0, and the DP value was between 0.471 1 and 0.997 0. A total of 497-species of haplotypes composed of the 10 loci were observed. Among them, there were 494 haplotypes with only one occurrence, and the HD value was 0.999 908 75 and DC was 0.994 0. Conclusion the fluorescent multiplex amplification system of 10 Y-STR loci is highly sensitive and has high genetic polymorphism in Henan Han population. It can be used in relevant research and application.
【作者单位】：新乡医学院法医学系法医物证学教研室;公安部物证鉴定中心;
【基金】：北京市科技计划项目(Z131100004513007) 河南省教育厅自然科学基金资助项目(13A310849) 新乡医学院培育基金重点项目(2013ZD114)
【分类号】：D919

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