跳跃负荷量对兔髌骨髌腱结合部的组织学及细胞凋亡的影响

发布时间：2018-12-11 06:25

【摘要】：目的:通过跳跃训练使髌骨髌腱结合部产生损伤,观察不同跳跃负荷积累量下兔骨腱结合部损伤的病理变化和细胞凋亡情况,探讨跳跃负荷量积累对兔骨腱结合部的组织学和细胞凋亡的影响,为探讨损伤机理提供理论依据。方法:雌性新西兰大白兔20只,分为跳跃2周、4周、6周、8周及对照组。采用跳跃装置进行跳跃训练,每周跳跃5天,每天跳跃150次,每周进行力学测试,得到主动起跳和自由落地峰值力大小。完成相应周数的跳跃训练后,处死、取材、常规组织学处理,行HE、Safranin O和免疫组化染色,用Meta Morph Premier对各组髌骨髌腱结合部细胞密度、纤维软骨带厚度、粘多糖蛋白面积等指标进行组织形态计量,免疫组化评定凋亡细胞的阳性染色细胞密度。显著性水平为P0.05。结果:实验所采用的跳跃训练方法可以使兔获得稳定的负荷。2W、4W组细胞密度非常显著高于CON组,6W组细胞密度比2W组显著降低,8W组细胞密度比2W组非常显著降低,6W、8W组细胞密度比4W均有非常显著降低;6W组的纤维软骨带厚度比CON组非常显著增加,6W组的纤维软骨带厚度比2W组显著增加,8W组纤维软骨带厚度比6W组显著降低;4W、6W组的粘多糖蛋白表达面积与CON组相比均有显著性差异,与6W相比有非常显著性差异,6W组粘多糖蛋白表达面积与2W相比有显著性差异;与CON组相比,跳跃各组Caspase-3表达均有非常显著的增加,8W组Caspase-3表达显著低于2W组、非常显著低于4W和6W组。结论:6周的跳跃积累量能使髌骨髌腱结合部的组织学和细胞凋亡发生最明显的变化,6周是此跳跃模型的重要时间节点;跳跃负荷能引起兔髌骨髌腱结合部细胞持续过度凋亡,细胞的持续凋亡过多和增殖不足是引起髌骨髌腱结合部损伤的机制之一。
[Abstract]:Objective: to observe the pathological changes and apoptosis of patellar tendon joint injury induced by jumping training. To investigate the effect of jumping load accumulation on the histology and apoptosis of rabbit bone tendon joint, and to provide a theoretical basis for the study of injury mechanism. Methods: twenty female New Zealand white rabbits were divided into 2 weeks, 4 weeks, 6 weeks, 8 weeks and control groups. The jump training is carried out by jumping device, jumping 5 days a week, jumping 150 times a day, and carrying out mechanical tests every week to obtain the peak force of active take-off and free landing. After the corresponding number of weeks of jumping training was completed, the animals were killed, the materials were collected, the routine histological treatment, HE,Safranin O and immunohistochemical staining were performed. The cell density of patella patellar tendon junction and the thickness of fibrous chondrocytes in each group were treated with Meta Morph Premier. The area of mucopolysaccharide protein was measured by histomorphometry, and the positive staining cell density of apoptotic cells was assessed by immunohistochemistry. The significant level was P0.05. Results: the jumping training method used in the experiment could make the rabbits gain stable load. The cell density in 2W 4W group was significantly higher than that in CON group, the cell density in 6W group was significantly lower than that in 2W group, the cell density in 8W group was significantly lower than that in 2W group, and the cell density in 8W group was significantly lower than that in 2W group. The cell density ratio of 8W group was significantly lower than that of 4W group. The thickness of fibrous chondrocytes in 6W group was significantly higher than that in CON group, the thickness of fibrous chondrocytes in 6W group was significantly higher than that in 2W group, and the thickness of fibrochondrocytes in 8W group was significantly lower than that in 6W group. (4) the area of mucopolysaccharide protein expression in the 6W group was significantly different from that in the CON group, and was significantly different from the 6W group, and the 6W group was significantly different from the 2W group. Compared with CON group, the expression of Caspase-3 in jumping group was significantly increased, the Caspase-3 expression in 8W group was significantly lower than that in 2W group, and was significantly lower than that in 4W and 6W groups. Conclusion: the histomorphology and apoptosis of patellar tendon junction can be changed most obviously by jumping accumulation at 6 weeks, and 6 weeks is an important time node of this jumping model. Jumping load can cause the patella patellar tendon junctional cells to continue to apoptosis. The excessive apoptosis and insufficient proliferation of the cells is one of the mechanisms of patellar tendon injury.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R87

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