极低频电磁场对小鼠血清趋化因子水平的影响

发布时间：2018-12-25 15:58

【摘要】：目的探讨极低频电磁场对小鼠血清趋化因子水平的影响。方法将130只健康4~6周龄SPF级雄性BALB/c小鼠随机分为5组,分别为空白(第0天)组(10只)及0(对照)、0.1、0.5、2.5 mT辐照组(各30只)。采用50 Hz极低频电磁场辐照小鼠,每天照射8 h;分别于第1、10、30天,测定小鼠血清中趋化因子单核细胞趋化蛋白-1(MCP-1)、嗜酸性粒细胞趋化因子(EOTAXIN-1)、MCP-3、巨噬细胞炎症蛋白-1α(MIP-1α)、MIP-1β、MIP-2、γ干扰素诱导的蛋白-10(IP-10)、生长相关癌基因-α(GRO-α)、正常T细胞表达分泌的活性调节蛋白(RANTES)的水平。结果与对照组相比,0.5 mT辐照第30天时小鼠血清MCP-1、GRO-α的水平及0.5、2.5 mT辐照第30天时小鼠血清MIP-1β水平以及各剂量辐照第10天、0.5 mT辐照第20天和0.1、0.5 mT辐照第30天时小鼠血清EOTAXIN-1的水平均较高,而0.5 mT辐照第10天时小鼠血清IP-10的水平和0.1 mT辐照第30天时小鼠血清RANTES的水平均较低,差异均有统计学意义(P0.05);各剂量辐照不同时间小鼠血清MCP-3、MIP-1α、MIP-2的水平均无明显改变。结论极低频电磁场对小鼠血清趋化因子MCP-1和EOTAXIN-1有刺激作用,EOTAXIN-1有可能作为一种稳定的生物标志物用于研究极低频电磁场对人体的影响。
[Abstract]:Objective to investigate the effect of very low frequency electromagnetic field on serum chemokine levels in mice. Methods 130 healthy SPF male BALB/c mice of 6 weeks old were randomly divided into 5 groups: blank (0 d) group (n = 10) and control group (n = 0). Mice were irradiated with 50 Hz very low frequency electromagnetic field for 8 hours per day. The levels of chemokine monocyte chemoattractant protein-1 (MCP-1), eosinophil chemokine (EOTAXIN-1), MCP-3, macrophage inflammatory protein-1 伪 (MIP-1 伪), MIP-1 尾 in serum of mice were measured on day 1, 10 ~ 30, respectively. MIP-2, 纬 -induced protein-10 (IP-10), growth-associated oncogene-伪 (GRO- 伪), and the level of (RANTES), a secreted active regulatory protein, were expressed in normal T cells. Results compared with the control group, the levels of serum MCP-1,GRO- 伪 at 30 days after 0. 5 mT irradiation, MIP-1 尾 levels in serum of 0. 5 and 2. 5 mT mice on the 30th day, and 10 days after irradiation with different doses were measured. The levels of serum EOTAXIN-1 were higher on the 20th day of 0.5 mT irradiation and on the 30th day of 0.1 mT irradiation. The level of serum IP-10 was lower on the 10th day of 0. 5 mT irradiation than that on the 30th day of 0. 1 mT irradiation, and the difference was statistically significant (P0.05). The levels of serum MCP-3,MIP-1 伪 and MIP-2 did not change significantly at different time after irradiation. Conclusion very low frequency electromagnetic field can stimulate serum chemokine MCP-1 and EOTAXIN-1 in mice. EOTAXIN-1 may be used as a stable biomarker to study the effect of very low frequency electromagnetic field on human body.
【作者单位】：南方医科大学公共卫生与热带医学院放射医学系;
【基金】：国家自然科学基金(81573076)
【分类号】：R818

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