不同负荷游泳运动对老龄大鼠心肌细胞形态及Bcl-2、Bax蛋白表达的影响

发布时间：2018-12-26 14:02

【摘要】：目的探讨不同负荷游泳运动对老龄大鼠心肌细胞中Bcl-2、Bax及HIF-1蛋白表达的影响及凋亡机制。方法将48只22月龄雄性健康Wistar老龄大鼠随机分为对照组(C)、大负荷组(H)、中等负荷组(M)和小负荷组(S),每组12只,进行为期8 w的不同负荷的游泳运动;运动干预后解剖取样,HE染色观察心肌细胞形态学变化,免疫组织化学检测心肌细胞Bcl-2、Bax和HIF-1蛋白表达。结果 S组老龄大鼠心肌中Bcl-2表达与C组(P0.01)差异非常显著,H组与C组(P0.05)差异显著,S组心肌Bax表达与C组(P0.01)差异非常显著,H组与C组(P0.05)差异显著,S组心肌细胞HIF-1α表达与C组(P0.05)差异显著,H组与C组(P0.01)差异非常显著。适宜的游泳运动(S组)心肌纤维排列有序,细胞间质减少,心肌纤维肥厚,走向正常,着色比较均匀,且未观察到肌丝断裂,也无充血坏死现象发生。S组促进Bcl-2表达增加,Bax蛋白表达降低,进而抑制心肌细胞凋亡发生;大负荷运动或不适宜(H、M组)利于Bcl-2蛋白的表达减少,Bax蛋白的表达升高,进而促进心肌细胞凋亡的发生。结论Bcl-2、Bax蛋白表达变化是运动干预心肌细胞凋亡的调控机制之一。
[Abstract]:Objective to investigate the effects of swimming under different loads on the expression of Bcl-2,Bax and HIF-1 protein in cardiac myocytes of aged rats and the mechanism of apoptosis. Methods Forty-eight healthy 22-month-old male Wistar rats were randomly divided into two groups: control group, (C), heavy load group, (H), medium load group, (M) group and small load group (S), 12 rats in each group. Swimming exercise with different loads for 8 weeks was carried out. The morphologic changes of cardiomyocytes were observed by HE staining and the expression of Bcl-2,Bax and HIF-1 protein were detected by immunohistochemistry. Results the expression of Bcl-2 in myocardium of aged rats in group S was significantly different from that in group C (P0.01), and that in group H and group C (P0.05). The expression of Bax in myocardium of group S was significantly different from that of group C (P0.01). The expression of HIF-1 伪 in group S was significantly higher than that in group C (P0.05), and the difference between group H and group C was very significant (P0.01). The suitable swimming exercise (group S) was characterized by orderly arrangement of myocardial fibers, decrease of interstitial cells, hypertrophy of myocardial fibers, normal direction, uniform coloration, and no myofilament breakage was observed. In S group, the expression of Bcl-2 was increased, the expression of Bax protein was decreased, and the apoptosis of cardiomyocytes was inhibited. Heavy load exercise or inappropriate exercise (Ham group) was beneficial to decrease the expression of Bcl-2 protein and increase the expression of Bax protein, thus promoting the occurrence of cardiomyocyte apoptosis. Conclusion the change of Bcl-2,Bax protein expression is one of the regulatory mechanisms of cardiomyocyte apoptosis induced by exercise.
【作者单位】：吉林大学体育学院;长春公安医院;日本早稻田大学;
【基金】：吉林省科技厅自然科学基金项目(201215037) 吉林省发改委产业技术研究与开发项目(No.2013C011) 吉林市科技局专项研究项目(No.2015313013)
【分类号】：R87

【相似文献】