大鼠一次性下坡跑运动后FNDC5和Irisin水平变化特点

发布时间：2019-01-11 12:55

【摘要】：目的:探讨大鼠一次性下坡跑运动后FNDC5和Irisin随时间的变化特点及意义。方法:30只8周龄Sprague-Dawley大鼠随机分为对照组(n=5)和一次性下坡跑运动组(n=25)。运动组大鼠在坡度为-16度的跑台上以17米/分的速度跑90分钟。在运动结束后的1天、3天、5天、7天和14天分别选取5只大鼠采集尾静脉血和比目鱼肌。通过ELISA检测大鼠血清Irisin水平;采用实时荧光定量PCR检测骨骼肌中FNDC5的表达;应用Western Blot检测骨骼肌中PGC1-α蛋白水平以及AMPK的磷酸化。结果:大鼠一次性下坡跑运动引起CK活性增加(运动后第5天,P0.05)和PGC1-α水平显著增加;FNDC5的m RNA水平在运动后的第1天和第3天升高(P0.05),随后降低;血清Irisin水平在运动后第1天增加(P0.05);同时,骨骼肌中AMPK激活。结论:一次性下坡跑运动引起PGC1-α表达增加,并在运动后初期通过调控大鼠骨骼肌表达FNDC5引起血清中Irisin含量增加,进而通过激活AMPK在运动后的骨骼肌能量代谢中发挥作用。
[Abstract]:Objective: to investigate the changes and significance of FNDC5 and Irisin after downhill running in rats. Methods: thirty 8 week old Sprague-Dawley rats were randomly divided into two groups: control group (n = 5) and once downhill running group (n = 25). The rats in the exercise group ran at a speed of 17 m / min for 90 minutes on the platform with-16 degrees slope. At 1 day, 3 days, 5 days, 7 days and 14 days after exercise, 5 rats were selected to collect blood from caudal vein and soleus muscle. The level of serum Irisin was detected by ELISA, the expression of FNDC5 in skeletal muscle was detected by real-time fluorescence quantitative PCR, the level of PGC1- 伪 protein and the phosphorylation of AMPK in skeletal muscle were detected by Western Blot. Results: the activity of CK and the level of PGC1- 伪 increased significantly in downhill running (P0.05) and the level of m RNA of FNDC5 increased on the 1st and 3rd day after exercise (P0.05) and then decreased. Serum Irisin level increased on the first day after exercise (P0.05), and AMPK was activated in skeletal muscle. Conclusion: once downhill running can increase the expression of PGC1- 伪, and increase the content of Irisin in serum by regulating the expression of FNDC5 in skeletal muscle of rats at the early stage of exercise, and then play a role in the energy metabolism of skeletal muscle after exercise by activating AMPK.
【作者单位】：国家体育总局体育科学研究所;国家体育总局反兴奋剂中心;
【基金】：国家体育总局体育科学研究所基本科研业务费资助项目(基本15-15)
【分类号】：R87

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