氚照射对大鼠海马神经细胞黏附分子L1、NCAM表达的影响

发布时间：2019-04-20 16:09

【摘要】：目的探讨低剂量氚照射对大鼠海马神经细胞黏附分子L1、神经细胞黏附分子(NCAM)表达的影响。方法取24 h新生鼠脑海马细胞培养,培养第7天以3.7×102、3.7×103、3.7×104、3.7×105、3.7×106Bq/ml终浓度氚水照射细胞24 h,并设对照组(0 Bq/ml),活细胞工作站测定各处理组细胞的迁移距离;Western blot法、免疫细胞化学法检测各浓度氚水照射下神经细胞黏附分子L1及NCAM蛋白的表达情况。结果神经细胞迁移距离测定结果显示,与对照组相比,受照组细胞迁移距离明显缩短,且随着氚水浓度的不断增加而逐渐缩短(P0.05);Western blot法、免疫细胞化学法检测显示受照组神经细胞黏附分子L1、NCAM蛋白表达含量均低于对照组,并随氚水浓度的升高表达量呈递减趋势(P0.05)。结论氚照射可致神经细胞黏附分子表达量减少进而可影响神经细胞的正常迁移。
[Abstract]:Aim to investigate the effects of low dose tritium irradiation on the expression of nerve cell adhesion molecule L1 and neural cell adhesion molecule (NCAM) in hippocampus of rats. Methods the cells were cultured for 24 h and irradiated with 3.7 脳 102,3.7 脳 103,3.7 脳 104,3.7 脳 105,3.7 脳 106Bq/ml final concentration of tritium water for 24 hours. The control group (0 Bq/ml) was set up. The migration distance of cells in each treatment group was measured by living cell workstation. Western blot method and immunocytochemical method were used to detect the expression of nerve cell adhesion molecule L1 and NCAM protein under various concentrations of tritiated water irradiation. Results compared with the control group, the migration distance of neurons in the irradiated group was significantly shorter than that in the control group, and gradually shortened with the increasing of the concentration of tritium water (P0.05). Western blot assay and immunocytochemistry showed that the expression of NCAM protein and L1 in the exposed group were lower than those in the control group, and the expression level decreased with the increase of tritium water concentration (P0.05). Conclusion tritium irradiation can decrease the expression of nerve cell adhesion molecules and then affect the normal migration of neural cells.
【作者单位】：安徽医科大学临床医学院放射医学研究所;安徽省立医院核医学科;芜湖市第二人民医院影像科;安徽省第二人民医院MRI室;肥东县人民医院呼吸科;
【基金】：国家自然科学基金(编号:81273003) 安徽省自然科学基金(编号:1208085MH162) 安徽省教育厅自然科学基金(编号:KJ2011Z162)
【分类号】：R811

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