有氧运动对慢性应激大鼠脑海马区神经可塑性的影响

发布时间：2019-04-21 17:44

【摘要】：目的:以神经元和神经胶质细胞相关神经生物学变化为切入点,探讨有氧运动对慢性应激大鼠脑海马区神经可塑性的影响。方法:以SD大鼠为研究对象,按照体重均衡分为对照组(CON)、慢性应激模型组(MOD)、氟西汀组(FLU)、低强度运动组(LIR)和中等强度运动组(MIR)。给予大鼠4周慢性应激,并分别伴随氟西汀给药、低强度或者中等强度的运动干预。4周后,通过开场实验、蔗糖饮水实验、新奇抑制摄食实验以及新物体识别实验检测有氧运动对慢性应激大鼠抑郁样行为、焦虑样行为以及认知障碍的影响;同时,利用酶联免疫法和蛋白免疫印迹法,检测有氧运动对慢性应激大鼠血清皮质酮、海马内前炎因子IL1-β,以及蛋白GFAP、S100β、BDNF、p-ERK、p-CREB表达的影响,探讨有氧运动抗抑郁的行为学效应和作用机制。结果:1)低强度和中等强度运动能够显著增加慢性应激大鼠的开场活动、缩短新奇抑制摄食潜伏期,同时提高慢性应激大鼠新物体的识别指数;2)有氧运动有效逆转了慢性应激大鼠血清皮质酮的异常升高,同时降低海马内前炎性因子IL1-β的分泌;3)有氧运动可显著提升胶质细胞的标志性功能蛋白GFAP和S100β的表达水平,增强慢性应激大鼠的BDNF/ERK/CREB信号转导通路中重要蛋白BDNF、p-ERK、pCREB的表达。结论:有氧运动可以通过提高慢性应激大鼠脑海马区神经可塑性来达到抗抑郁的目的。可能与以下因素有关:1)有氧运动调节血清皮质酮的异常升高,降低海马内前炎性因子的释放,同时,提高与神经胶质细胞相关蛋白GFAP和S100β活性,从而起到神经元保护的作用;2)有氧运动可以通过激活CREB、BDNF以及ERK等重要信号蛋白的表达,提高神经营养作用,促进神经元的生长,增加神经可塑性和神经发生来发挥抗抑郁作用。
[Abstract]:Aim: to investigate the effects of aerobic exercise on neuroplasticity in the cerebral equine region of rats with chronic stress, based on the neurobiological changes associated with neurons and glial cells. Methods: SD rats were divided into two groups according to body weight balance: control group (CON), chronic stress model group (MOD), fluoxetine group (FLU), low intensity exercise group (LIR) and moderate intensity exercise group (MIR). Rats were given chronic stress for 4 weeks with fluoxetine administration, low or medium intensity exercise intervention. 4 weeks later, through the open field experiment, sucrose drinking test, the experimental results were as follows: (1) after 4 weeks, the rats were treated with fluoxetine for 4 weeks. The effects of aerobic exercise on depression-like behavior, anxiety-like behavior and cognitive impairment in chronic stress rats were detected by novel inhibition feeding test and new object recognition test. At the same time, the effects of aerobic exercise on the expression of serum corticosterone, IL1- 尾, GFAP,S100 尾 and BDNF,p-ERK,p-CREB in hippocampus of chronic stress rats were detected by enzyme linked immunosorbent assay (Elisa) and protein immunoblotting. To explore the behavioral effect and mechanism of aerobic exercise in anti-depression. Results: 1) low-intensity and moderate-intensity exercise could significantly increase the opening activity of chronic stress rats, shorten the novel inhibition of feeding latency, and increase the recognition index of new objects in chronic stress rats. 2) aerobic exercise can effectively reverse the abnormal increase of serum corticosterone and decrease the secretion of proinflammatory factor IL1- 尾 in the hippocampus of chronic stress rats. 3) aerobic exercise significantly increased the expression of GFAP and S100 尾 in glial cells, and increased the expression of BDNF,p-ERK,pCREB in the BDNF/ERK/CREB signal transduction pathway of chronic stress rats. Conclusion: aerobic exercise can improve neuroplasticity in cerebral equine region of chronic stress rats to achieve the purpose of anti-depression. It may be related to the following factors: 1) aerobic exercise regulates the abnormal elevation of serum corticosterone, reduces the release of proinflammatory factors in the hippocampus, and increases the activity of glial cell-associated proteins GFAP and S100 尾. Thus play the role of neuron protection; 2) aerobic exercise can play an antidepressant role by activating the expression of important signal proteins such as CREB,BDNF and ERK, enhancing neurotrophic effect, promoting the growth of neurons, increasing neuroplasticity and neurogenesis.
【作者单位】：北京体育大学运动康复系;解放军总医院康复医学中心;军事医学科学院;
【基金】：高等学校博士学科点专项科研基金(编号:20111112120002)
【分类号】：R87

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