射线照射大鼠肾放射损伤的实验研究
发布时间：2019-05-31 15:21
【摘要】：目的：从肾功能、肾组织病理方面观察大鼠肾脏接受照射后的改变,探讨不同剂量电子线单次照射大鼠肾脏产生的放射生物学效应,为腹部放疗的临床应用提供基础参数。 方法：120只雄性Sprague-Dawley (SD)大鼠随机分为健康对照组、8Gy照射组、10Gy照射组、12Gy照射组及15Gy照射组,每组24只。用10%水合氯醛(0.3m1/100g)注射大鼠腹腔麻醉,将其俯卧于大鼠特制照射模具上,将大鼠四肢拉伸固定。,置于模拟机下定位以确定照射范围,探及两侧肾脏具体位置,于体表标记照射野,限光筒限定照射野大小为2.5cm×2.5cm的方野。将定位好的大鼠置于Siemens Primus M直线加速器下,采用5MeV的电子线照射双侧肾脏,照射剂量分别为8Gy、10Gy、12Gy、15Gy。剂量率为300cGy/min,源皮距(Sourse skin distance,SSD)为100cm。对照组麻醉后佯装照射。 测定大鼠放射结束后第1、3、6、9月的肾脏系数(kidney coefficient)、收缩压(SBP)、血浆胱抑素C (CysC)、血尿素氮(BUN)、血清肌酐(Scr)、尿蛋白/尿肌酐(UP/UC)的变化。HE染色观察大鼠肾脏的病理形态学改变。 结果：与健康对照组相比,8Gy及以上照射组的肾脏系数照射后9个月均显著变小(P0.01)；15Gy照射组SBP于照射后9个月显著升高(P0.01)；Scr、BUN：12Gy及以上照射组于6个月起显著增高(P0.01),8Gy、10Gy组于9个月显著增高(P0.01)。UP/UC:15Gy组于照射后3月起开始显著升高(P0.01)；8Gy、10Gy、12Gy组于6个月起开始显著升高(P0.01)。CysC:10Gy及以上组照射后6个月起显著升高(P0.01),8Gy组于9个月显著升高(P0.01)。于9个月时各照射组均可观察到不同程度肾脏体积缩小,皮质比例减小。肾脏组织病理学显示：光镜下观察可见,空白对照组肾脏结构清晰,皮质、髓质结构正常,肾小球、肾小管及间质均无异常改变。各照射组均观察到不同程度肾脏体积缩小,皮质比例减小,系膜细胞溶解,后期呈现硬化趋势。其中15Gy组硬化趋势最为明显。系膜基质与上皮细胞粘连,肾小球毛细血管袢增厚,血管腔扩大,肾小管上皮细胞间隙增大,部分上皮细胞肿胀、溶解、坏死。细胞基膜明显增厚。8Gy组9月病理变化较轻微,以系膜增生为主。15Gy组(3月开始)、12Gy组(6月开始)可观察到肾小管间质纤维化改变,但基本无炎性细胞浸润。9月时各照射组均可观察到不同程度的血栓形成,随剂量升高程度递增。 结论：大鼠双肾接受单次不同剂量电子线体外照射可引起不同程度肾脏形态及功能损伤,且损伤程度与损伤出现时间与照射剂量相关,即一定范围内(8Gy-15Gy)照射剂量越小所造成的肾脏损伤程度越轻,出现时间越晚。
[Abstract]:Aim: to observe the changes of kidney after irradiation in rats from the aspects of renal function and renal histology, and to explore the radiobiological effects of single irradiation with different doses of electron rays, so as to provide basic parameters for the clinical application of abdominal radiotherapy. Methods: 120 male Sprague-Dawley (SD) rats were randomly divided into healthy control group, 8Gy irradiation group, 10Gy irradiation group, 12Gy irradiation group and 15Gy irradiation group with 24 rats in each group. The rats were anesthetized intraabdominal with 10% chloral hydrate (0.3m1/100g). The rats were prone to lie on the special irradiation mould of the rats, and the limbs of the rats were stretched and fixed. The rats were positioned under the simulator to determine the irradiation range, and to explore the specific position of the kidneys on both sides. The irradiation field was marked on the body surface, and the size of the irradiation field was limited to the square field of 2.5cm 脳 2.5cm. The localized rats were exposed to Siemens Primus M linear accelerator. The bilateral kidneys were irradiated with 5MeV electron lines at doses of 8, 10 and 12, respectively. The dose rate was 300 cGY / min and the distance between source skin and (Sourse skin distance,SSD was 100 cm. The control group pretended irradiation after anesthesia. Renal coefficient (kidney coefficient), systolic blood pressure (SBP), plasma cystine C (CysC), blood urea nitrogen (BUN), serum creatine (Scr), were measured at the 1st, 3rd, 6th and 9th month after radiation in rats. The changes of urinary protein / urinary creatinine (UP/UC). The pathomorphological changes of kidney in rats were observed by HE staining. Results: compared with the healthy control group, the renal coefficient of 8Gy and above groups decreased significantly at 9 months after irradiation (P 0.01), and the SBP of 15Gy irradiation group increased significantly at 9 months after irradiation (P 0.01). The levels of Scr,BUN:12Gy and above in the irradiation group increased significantly from 6 months (P 0.01), and those in the 10 GY group increased significantly at 9 months (P 0.01). The levels in the UP/UC:15Gy group began to increase significantly from 3 months after irradiation (P 0.01). The levels of CysC: 10Gy and above increased significantly from 6 months after irradiation (P 0.01), and those in 8Gy group increased significantly at 9 months after irradiation (P 0.01). At 9 months, the volume of kidney decreased and the proportion of cortex decreased in all irradiation groups. Renal histology showed that under light microscope, the renal structure of the blank control group was clear, the cortex and medulla structure were normal, and there were no abnormal changes in glomeruli, tubules and stroma. In each irradiation group, the volume of kidney decreased, the proportion of cortex decreased, the Mesangial cells dissolved, and showed a trend of sclerosis in the later stage. Among them, the hardening trend was the most obvious in 15Gy group. Mesangial matrix adheres to epithelial cells, thickening of glomerular capillary loop, enlargement of vascular cavity, enlargement of renal tubular epithelial cell space, swelling, dissolution and necrosis of some epithelial cells. The pathological changes of the cell basement membrane were mild in the 8GY group in September, mainly Mesangial hyperplasia. The renal tubulointerstitial fibrosis was observed in the 15GY group (from 3 months) and the 12Gy group (from 6 months). However, there was no inflammatory cell infiltration. Thrombus formation was observed in all irradiation groups at 9 months, and increased with the increase of dose. Conclusion: single different doses of electron beam irradiation can cause different degrees of renal morphological and functional damage in rats, and the degree of injury is related to the time of injury and the dose of irradiation. That is to say, the smaller the dose of 8Gy-15Gy, the lighter the degree of renal injury and the later the occurrence time.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R730.55
本文编号：2489806
[Abstract]:Aim: to observe the changes of kidney after irradiation in rats from the aspects of renal function and renal histology, and to explore the radiobiological effects of single irradiation with different doses of electron rays, so as to provide basic parameters for the clinical application of abdominal radiotherapy. Methods: 120 male Sprague-Dawley (SD) rats were randomly divided into healthy control group, 8Gy irradiation group, 10Gy irradiation group, 12Gy irradiation group and 15Gy irradiation group with 24 rats in each group. The rats were anesthetized intraabdominal with 10% chloral hydrate (0.3m1/100g). The rats were prone to lie on the special irradiation mould of the rats, and the limbs of the rats were stretched and fixed. The rats were positioned under the simulator to determine the irradiation range, and to explore the specific position of the kidneys on both sides. The irradiation field was marked on the body surface, and the size of the irradiation field was limited to the square field of 2.5cm 脳 2.5cm. The localized rats were exposed to Siemens Primus M linear accelerator. The bilateral kidneys were irradiated with 5MeV electron lines at doses of 8, 10 and 12, respectively. The dose rate was 300 cGY / min and the distance between source skin and (Sourse skin distance,SSD was 100 cm. The control group pretended irradiation after anesthesia. Renal coefficient (kidney coefficient), systolic blood pressure (SBP), plasma cystine C (CysC), blood urea nitrogen (BUN), serum creatine (Scr), were measured at the 1st, 3rd, 6th and 9th month after radiation in rats. The changes of urinary protein / urinary creatinine (UP/UC). The pathomorphological changes of kidney in rats were observed by HE staining. Results: compared with the healthy control group, the renal coefficient of 8Gy and above groups decreased significantly at 9 months after irradiation (P 0.01), and the SBP of 15Gy irradiation group increased significantly at 9 months after irradiation (P 0.01). The levels of Scr,BUN:12Gy and above in the irradiation group increased significantly from 6 months (P 0.01), and those in the 10 GY group increased significantly at 9 months (P 0.01). The levels in the UP/UC:15Gy group began to increase significantly from 3 months after irradiation (P 0.01). The levels of CysC: 10Gy and above increased significantly from 6 months after irradiation (P 0.01), and those in 8Gy group increased significantly at 9 months after irradiation (P 0.01). At 9 months, the volume of kidney decreased and the proportion of cortex decreased in all irradiation groups. Renal histology showed that under light microscope, the renal structure of the blank control group was clear, the cortex and medulla structure were normal, and there were no abnormal changes in glomeruli, tubules and stroma. In each irradiation group, the volume of kidney decreased, the proportion of cortex decreased, the Mesangial cells dissolved, and showed a trend of sclerosis in the later stage. Among them, the hardening trend was the most obvious in 15Gy group. Mesangial matrix adheres to epithelial cells, thickening of glomerular capillary loop, enlargement of vascular cavity, enlargement of renal tubular epithelial cell space, swelling, dissolution and necrosis of some epithelial cells. The pathological changes of the cell basement membrane were mild in the 8GY group in September, mainly Mesangial hyperplasia. The renal tubulointerstitial fibrosis was observed in the 15GY group (from 3 months) and the 12Gy group (from 6 months). However, there was no inflammatory cell infiltration. Thrombus formation was observed in all irradiation groups at 9 months, and increased with the increase of dose. Conclusion: single different doses of electron beam irradiation can cause different degrees of renal morphological and functional damage in rats, and the degree of injury is related to the time of injury and the dose of irradiation. That is to say, the smaller the dose of 8Gy-15Gy, the lighter the degree of renal injury and the later the occurrence time.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R730.55
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相关期刊论文 前3条
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