模拟失重大鼠肝组织中NF-κB的表达及意义

发布时间：2018-01-29 21:02

本文关键词： 模拟失重大鼠肝脏核因子κB 蛋白免疫印迹法免疫组化　出处：《世界华人消化杂志》2008年31期 　论文类型：期刊论文

【摘要】：目的:研究模拟失重环境下大鼠肝脏组织中NF-κB的表达及意义.方法:成年♂Wistar大鼠84只,随机分为模拟失重组和对照组,每组又分别设1、2、3、4、5、6和7d共7个时相点,每时相点模拟失重和同步对照各6只大鼠.采用尾悬吊法建立模拟失重动物模型.各组大鼠肝组织中NF-κB表达分别应用Western blot和免疫组化PV-6001法进行检测.结果:在尾悬吊1-2d期间,大鼠焦躁不安,饮食量减少,精神较差,活动减弱,2-3d后有所适应,逐渐恢复稳定状态.模拟失重环境下大鼠肝脏NF-κB表达水平明显升高,1、2d悬尾组大鼠肝组织中NF-κB表达率明显高于对照组(F=271.36,P0.01),其后,NF-κB表达水平呈明显下降趋势(F=60.68,P0.05),5-7d悬尾组NF-κB表达水平接近对照组,差异无统计学意义.NF-κB阳性产物主要见于实验大鼠肝细胞内,亦见于炎细胞及Kupffer细胞内,可分为胞质型、核型、核浆型等三个类型,单独或混合存在.结论:模模拟失重使大鼠肝脏组织中NF-κB表达发生明显变化,提示在失重环境中肝脏NF-κB的早期高表达和逐渐恢复过程与失重应激反应及失重耐受有密切关系.
[Abstract]:Objective: to study the expression and significance of NF- 魏 B in rat liver under simulated weightlessness. 鈾侲ighty-four Wistar rats were randomly divided into two groups: the control group and the simulated group. Each group was divided into 7 time points at 1, 2, 3, 4, 5, 5, 6 and 7 days, respectively. The animal model of simulated weightlessness was established by tail suspension method. The expression of NF- 魏 B in liver tissues of each group was treated with Western, respectively. Blot and immunohistochemical PV-6001 method were used. Results: during 1-2 days of tail suspension. The rats were restless, diet decreased, mental health was poor, the activity decreased 2-3 days later, and gradually recovered to stable state. The expression of NF- 魏 B in the liver of rats was significantly increased under simulated weightlessness environment. 2-3 days later, the expression level of NF- 魏 B in the liver of rats was significantly increased. The expression rate of NF- 魏 B in the liver tissue of the tail suspension group was significantly higher than that of the control group (P 0.01). The expression of NF- 魏 B in the tail suspension group was similar to that in the control group. The positive products of NF- 魏 B were mainly found in the hepatocytes of experimental rats, but also in inflammatory cells and Kupffer cells, which could be divided into three types: cytoplasmic type, karyotypic type and nuclear cytoplasmic type. Conclusion: simulated weightlessness can significantly change the expression of NF- 魏 B in rat liver. The results suggest that the early high expression and gradual recovery of NF- 魏 B in the liver are closely related to weightlessness stress response and weightlessness tolerance.
【作者单位】：中国人民解放军第三军医大学西南医院全军肝胆外科研究所;中国人民解放军第306医院普通外科;中国人民解放军第306医院病理科;
【基金】：全军“11.5”计划面上基金资助项目,No.06MA005~~
【分类号】：R85
【正文快照】： ■背景资料机体在失重环境中受到瀑布式的直接和间接的失重应激作用,正常生理机能受到影响.近年来,我国航天事业迅猛发展,航天员特殊训练和载人航天飞行的成功及后续重大任务对航天医学提出了新的要求,但迄今国内尚未见有关失重和模拟失重应激环境下肝脏生理和病理研究的报道

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