沙漠干热环境下猪腹部肠管火器伤后肝细胞凋亡和肝功能变化

发布时间：2018-02-01 04:42

本文关键词： 腹部火器伤肝损伤凋亡沙漠干热环境　出处：《世界华人消化杂志》2008年11期 　论文类型：期刊论文

【摘要】：目的:观察沙漠干热环境下猪腹部肠管火器伤后肝细胞凋亡和肝脏功能的变化,并探讨肝细胞凋亡和肝脏损害的关系.方法:沙漠干热环境组和常温环境组各健康长白仔猪42头随机等分为对照组和伤后1、2、4、8、12和24h组,实验组建立腹部肠管火器伤动物模型后,采用TUNEL法观察肝细胞凋亡情况,同时测定各时间点血清ALT水平.结果:伤后各组肝细胞凋亡指数明显高于对照组(沙漠干热环境:21.18%±2.23%,49.51%±4.18%,42.61%±3.20%,76.47%±3.64%72.26%±4.98%,61.41%±3.98%vs2.98%±0.74%,P0.05;常温环境:60.37%±3.99%73.40%±2.93%,48.97%±1.73%,32.17%±1.50%,42.92%±1.98%,19.04%±0.72%vs2.65%±0.37%,P0.05),并于伤后2h出现第1个高峰,沙漠干热环境组和常温环境组分别于伤后8h和12h出现第2个高峰;血清ALT的变化趋势与肝细胞凋亡的趋势一致;在沙漠干热环境和常温环境下,肝细胞凋亡指数和ALT均呈正相关(r=0.91,0.93;均P0.01).结论:肝细胞凋亡在肝脏的损害过程中起重要作用,干预肝细胞凋亡可能是腹部肠管火器伤后减少肝损伤的有效措施.
[Abstract]:Objective: to observe the changes of hepatocyte apoptosis and liver function after gunshot injury of abdominal intestine in pigs in desert dry and hot environment. Methods: 42 healthy Landrace piglets in desert dry and hot environment and normothermic environment were randomly divided into control group and control group. After the experimental group established the animal model of abdominal gunshot injury, the apoptosis of hepatocytes was observed by TUNEL method. Results: the apoptotic index of hepatocytes in each group was significantly higher than that in control group (21.18% 卤2.23% in desert dry heat environment). 49.51% 卤4.18% 42.61% 卤3.20% and 76.47% 卤3.64r.26% 卤4.98%. 61.41% 卤3.98% vs 2.98% 卤0.74% P 0.05; At normal temperature: 60.37% 卤3.99 s.40% 卤2.93% + 48.97% 卤1.73% and 32.17% 卤1.50% 卤42.92% 卤1.98%. 19.04% 卤0.72 vs 2.65% 卤0.37%, and the first peak occurred at 2 h after injury. The second peak appeared at 8 h and 12 h after injury in desert dry hot environment group and normal temperature environment group respectively. The change trend of serum ALT was consistent with that of hepatocyte apoptosis. The apoptotic index of hepatocytes was positively correlated with ALT in desert dry hot environment and normal temperature environment. Conclusion: apoptosis of hepatocytes plays an important role in the process of liver injury. Intervention of hepatocyte apoptosis may be an effective measure to reduce liver injury after abdominal gunshot injury.
【作者单位】：中国人民解放军兰州军区乌鲁木齐总医院普外科中国人民解放军兰州军区乌鲁木齐总医院普外科中国人民解放军兰州军区乌鲁木齐总医院普外科中国人民解放军兰州军区乌鲁木齐总医院动物实验中心中国人民解放军兰州军区乌鲁木齐总医院医务部中国人民解放军兰州军区乌鲁木齐总医院普外科中国人民解放军兰州军区乌鲁木齐总医院医务部
【基金】：中国人民解放军兰州军区医药卫生科研基金资助项目,No.LXH-2005019~~
【分类号】：R826
【正文快照】： 0引言腹部火器伤创伤机制的研究较少,沙漠干热环境下腹部火器伤的研究尚属国际研究领域的空白.腹部火器伤具有肠穿孔率高、多脏器功能失调综合征(multiorgan dysfunction syndrome,MODS)发生率高的特点,肠道是机体最大的细菌及毒素储库,是MODS的始动器官[1].炎症过程的失调和

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