模拟失重对大鼠腹主动脉L-Arg-NO-cGMP通路的影响

发布时间：2018-02-10 01:32

本文关键词： 模拟失重一氧化氮合酶大鼠主动脉　出处：《中国应用生理学杂志》2006年03期 　论文类型：期刊论文

【摘要】：目的:观察尾部悬吊模拟失重对大鼠腹主动脉舒张反应性与一氧化氮合酶表达的影响。方法:体重300~330 g的20只雄性SD大鼠按体重配对随机分为对照组与模拟失重组,模拟失重大鼠采用尾部悬吊方法模拟失重。4周后,利用离体动脉血管环舒张实验与Western blot蛋白免疫印迹方法观察了腹主动脉舒张反应性和腹主动脉一氧化氮合酶eNOS(endothelial NOS)和iNOS(inducible NOS)的表达。结果:悬吊大鼠腹主动脉环对左旋精氨酸(L-Arg)与乙酰胆碱(Ach)的舒张反应显著低于对照,而对硝普钠(SNP)与环磷酸鸟苷(cGMP)的舒张反应在两组间无显著不同。其敏感性在两组间均无显著差别。腹主动脉的eNOS与iNOS表达在模拟失重组与对照组间亦未发现显著差别。结论:本工作提示尾部悬吊模拟失重下大鼠腹主动脉舒张反应的减弱可能是动脉血管内皮功能改变的结果,尤其是NOS活性的变化可能更为重要。
[Abstract]:Objective: to observe the effect of tail suspension simulated weightlessness on diastolic reactivity and nitric oxide synthase expression in abdominal aorta of rats. Methods: twenty male SD rats weighing 300 ~ 330g were randomly divided into control group and simulated loss group according to their body weight. The tail suspension method was used to simulate weightlessness in simulated weightlessness rats after 4 weeks of weightlessness. The vasodilation reaction of abdominal aorta and the expression of nitric oxide synthase (eNOS(endothelial NOSs) and iNOS(inducible NOSs in abdominal aorta were observed by Western blot Western blotting method and in vitro. The diastolic response of L-Arg to acetylcholine Ach) was significantly lower than that of the control. There was no significant difference between the two groups in the diastolic response to sodium nitroprusside and guanosine cyclophosphate. There was no significant difference in sensitivity between the two groups. The expression of eNOS and iNOS in abdominal aorta was not significantly different between the two groups. Conclusion: this work suggests that the decrease of aortic relaxation in rats under simulated weightlessness may be the result of changes in vascular endothelial function. In particular, the change of NOS activity may be more important.
【作者单位】：第四军医大学航空航天生理学教研室西京医院呼吸内科第四军医大学航空航天生理学教研室第四军医大学航空航天生理学教研室第四军医大学航空航天生理学教研室
【基金】：国家自然科学基金资助课题(30170355)
【分类号】：R852.22

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