不同类型射线对人淋巴细胞染色体畸变影响

发布时间：2018-02-14 19:01

本文关键词： ~()Fe~(+)重离子束 ~()C~(+)重离子束 ~()Co γ射线人淋巴细胞染色体畸变双着丝粒体着丝粒环　出处：《中国职业医学》2017年03期 　论文类型：期刊论文

【摘要】：目的比较铁-56(~(56)Fe~(17+))、碳-12(~(12)C~(6+))重离子束与钴-60(~(60)Co)γ射线对人淋巴细胞染色体畸变的影响。方法将人淋巴细胞按照射剂量分为0.1、0.3、0.5、0.7、1.0、2.0 Gy照射组和0.0 Gy对照组,分别采用~(56)Fe~(17+)(传能线密度为400.0 ke V/μm)、~(12)C~(6+)(传能线密度为26.0 ke V/μm)重离子束和~(60)Coγ射线进行照射,照后48 h收获细胞,采用光学显微镜人工计数法检测人淋巴细胞染色体双着丝粒体加着丝粒环(以下简称"双+环")畸变率和畸变细胞率。结果人淋巴细胞经~(56)Fe~(17+)、~(12)C~(6+)重离子束和~(60)Co γ射线照射后,0.3~2.0 Gy照射组~(12)C~(6+)重离子束诱导的"双+环"畸变率均高于相同剂量组的~(56)Fe~(17+)重离子束和~(60)Co γ射线(P0.05),0.1~2.0 Gy照射组~(12)C~(6+)重离子束诱导的畸变细胞率均高于相同剂量组的~(56)Fe~(17+)重离子束和~(60)Coγ射线(P0.05)。3种射线诱导的人淋巴细胞畸变效应在0.0~2.0 Gy内均呈随剂量增加而升高的趋势(P0.01)。~(56)Fe~(17+)重离子束的相对生物效应值低于相同剂量的~(12)C~(6+)重离子束。结论~(12)C~(6+)重离子束导致的人淋巴细胞染色体畸变较~(60)Coγ射线和~(56)Fe~(17+)重离子束严重。
[Abstract]:Objective to compare the effects of heavy ion beam and 纬 -ray on chromosome aberration of human lymphocytes. Methods Human lymphocytes were divided into 0. 1 ~ 0. 3 ~ 0. 5 ~ 0. 0 ~ 0. 0 Gy irradiation group and 0. 0 Gy control group. The cells were harvested 48 h after irradiation with heavy ion beam (energy transfer line density of 400.0 Kev / 渭 m) and heavy ion beam (energy transfer line density of 26.0 KV / 渭 m). The rate of aberration and the rate of aberrant cells of human lymphocyte chromosome bicentric granulocyte plus centromere ring (hereafter referred to as "double loop") were measured by means of optical microscope artificial counting method. Results the results showed that human lymphocytes were subjected to heavy ion beam and 60Co 纬. The rate of "double ring" aberration induced by heavy ion beam was higher than that of the same dose group (0. 3 ~ 2. 0 Gy group). The rate of "double ring" aberration induced by heavy ion beam was higher than that of the same dose group.) the rate of aberration cells induced by heavy ion beam was higher than that of phase group (P 0. 05 ~ 0. 0 ~ 2. 0 Gy group). The rate of aberrant cells induced by heavy ion beam was higher than that of phase group. In the same dose group, the relative biological effect of heavy ion beam and 60Co 纬 -ray irradiation P0.053.The aberration effect of human lymphocytes increased with the increase of dose in 0.020 Gy. The relative biological effect of heavy ion beam was lower than that of the same dose. Conclusion the chromosome aberration of human lymphocytes induced by heavy ion beam is more serious than that caused by 60Co 纬 -ray and 560Co 纬 -ray.
【作者单位】：中国辐射防护研究院放射医学与环境医学研究所;
【分类号】：R85

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