贫铀片嵌入大鼠肌肉对骨代谢和骨矿盐损伤效应研究

发布时间：2018-02-15 15:40

本文关键词： 贫铀慢性污染骨损伤骨密度生物力学骨形态计量学　出处：《复旦学报(医学版)》2007年06期 　论文类型：期刊论文

【摘要】：目的探讨贫铀(depleted uranium,DU)嵌入大鼠肌肉致体内长期内污染是否会引起机体骨代谢和骨矿盐结构的损伤及其生物学特点,为DU创伤采取的早期医学处理方法和预防DU所致远期危害提供理论依据。方法采用DU片植入大鼠腓肠肌以建立DU嵌入损伤致DU长期内污染的动物模型,电感耦合等离子体质谱仪(inductive coupled plasma-mass spectrometry,ICP-MS)分析植入后不同时间点铀在骨组织中的分布和滞留规律;并在DU植入后3个月测定腰椎和股骨的骨密度、骨生物力学性能,光镜和电镜观察大鼠骨组织结构病理改变并进行形态计量学分析。结果随着植入DU剂量的不同,铀在骨骼组织中的分布浓度呈明显差异,且随着植入时间的延长,组织中的铀含量持续升高,至植入后90d达到最高峰。此后,骨骼中的铀含量开始逐渐下降,但下降速度缓慢,植入后360d骨骼中的铀含量仍维持在较高水平。DU植入后3个月,骨组织结构出现明显损伤,表现为骨小梁体积和骨小梁宽度减少、骨小梁间距增加、MAR值降低。植入DU大鼠的腰椎BMD明显降低,腰椎压缩和股骨三点弯最大载荷明显下降。结论骨骼是DU嵌入损伤后的主要蓄积器官,且滞留时间较长。DU长期内污染可引起骨量值明显降低、骨生物力学性能下降、骨组织结构出现明显损伤。应重视DU长期内污染对骨骼的损伤效应研究。
[Abstract]:Objective to investigate whether the long-term contamination caused by depleted uranium depleted uraniumum du (DU) embedded into rat muscles will cause damage to bone metabolism and bone mineral structure and its biological characteristics. Methods DU was implanted into the gastrocnemius muscle of rats to establish an animal model of long-term DU contamination caused by DU embedding injury. Inductively coupled plasma mass spectrometer (ICP-MS) was used to analyze the distribution and retention of uranium in bone tissue at different time points after implantation, and the bone mineral density (BMD) and bone biomechanical properties of lumbar vertebrae and femur were measured 3 months after DU implantation. The histopathological changes of bone tissue in rats were observed by light microscope and electron microscope. Results with the different doses of DU implanted, the concentration of uranium in bone tissue was significantly different, and with the prolongation of implantation time, the distribution of uranium in bone tissue was significantly different. The content of uranium in the tissue continued to increase, reaching its peak 90 days after implantation. After that, the content of uranium in the bone began to decrease gradually, but slowly, and the uranium content in the bone remained at a high level 3 months after implantation at the 360th day after implantation. The bone structure was obviously damaged, such as the reduction of trabecular volume and trabecular width, the increase of trabecular spacing and the decrease of BMD in lumbar vertebrae of DU rats. Conclusion Bone is the main accumulative organ after DU embedded injury, and the long-term contamination of DU can significantly decrease the bone mass and the biomechanical properties of bone. We should pay more attention to the damage effect of DU contamination on bone.
【作者单位】：复旦大学放射医学研究所放射卫生部上海市疾病预防控制中心毒理研究室复旦大学放射医学研究所放射卫生部复旦大学放射医学研究所放射卫生部上海市疾病预防控制中心毒理研究室
【基金】：国家自然科学基金项目(30370442)资助
【分类号】：R82

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