局部海水浸泡对SMIR模型大鼠疼痛行为学和继发神经病理改变的影响

发布时间：2018-02-20 02:04

本文关键词： 海水浸泡疼痛超微结构自噬　出处：《解放军医学杂志》2016年07期 　论文类型：期刊论文

【摘要】：目的评价局部海水浸泡对皮肤/肌肉切口牵拉痛(SMIR)模型大鼠疼痛行为学及继发神经病理改变的影响。方法选择健康成年雄性SD大鼠48只,随机分为模型对照组(MC组,只建立SMIR模型,不予浸泡处理)、生理盐水浸泡组(NS组,建立模型后局部伤口给予生理盐水浸泡1h)、海水浸泡组(SW组,建立模型后局部伤口给予海水浸泡1h),于术前1d及术后1、3、5、7、14、21、28d测定机械缩足反射阈值(MWT),于术后3、28d各组分别随机取8只大鼠,取术侧隐神经行HE染色、取术侧L3及L4背根神经节应用电镜技术观察其超微结构变化。结果 SW组术后各时点MWT值均明显低于NS组和MC组,且至术后28d仍明显低于术前基础水平;NT组和NS组术后各时点MWT值无明显差异,两组术后28d的MWT值与术前基础水平相当。SW组术后3、28d隐神经HE染色显示均有不同程度的神经损伤表现,而MC组和NS组未见异常;术后3、28d电镜观察见SW组背根神经节神经元内有大量空泡样线粒体和自噬小体,MC组和NS组未见明显病理改变。结论海水浸泡局部伤口可加重SMIR模型大鼠的急性疼痛,并可能导致其疼痛向慢性化转变,海水浸泡导致的外周神经损伤、背根神经节神经元超微结构改变可能参与了其疼痛行为学改变的机制。
[Abstract]:Objective to evaluate the effects of local seawater immersion on pain behavior and secondary neuropathological changes in skin / muscle incision stretch pain rats. Methods 48 healthy adult male SD rats were randomly divided into model control group and control group. The SMIR model was established only. The saline immersion group was treated with NS group. After the model was established, the local wound was given saline immersion for 1 hour, and the seawater immersion group was given the SW group. After the model was established, the local wound was treated with seawater immersion for 1 hour. The mechanical foot reflex threshold (MWTT) was measured at 1 day before operation and 1 day after operation. Eight rats were randomly selected from each group on the 3rd and 28th days after operation. The saphenous nerve of the operative side was stained with HE. The ultrastructural changes of L3 and L4 dorsal root ganglion were observed by electron microscopy. Results the MWT values in SW group were significantly lower than those in NS group and MC group at all time points after operation. At 28 days after operation, the MWT values of NT group and NS group were still significantly lower than those of preoperative basic level NT group and NS group, and there was no significant difference at each time point between NT group and NS group. The MWT values of the two groups on the 28th day after operation were similar to the basic level before operation. The HE staining of the saphenous nerve in the SW group showed different degrees of nerve injury on the 38th day after operation, but there was no abnormality in the MC group and NS group. A large number of vacuolated mitochondria and autophagy bodies in the neurons of dorsal root ganglion neurons in group SW were observed by electron microscope on the 328th day after operation. There were no obvious pathological changes in MC group and NS group. Conclusion the acute pain of SMIR model rats can be aggravated by seawater immersion in local wound. It may lead to the transformation of pain to chronic, the peripheral nerve injury caused by seawater immersion, and the ultrastructural changes of dorsal root ganglion neurons may be involved in the mechanism of pain behavior changes.
【作者单位】：广州军区广州总医院麻醉科全军临床麻醉中心;
【基金】：全军医学科研“十二五”科技攻关项目(BWS11J016)~~
【分类号】：R82

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