晕船适应大鼠脑干差异表达蛋白质的筛选及鉴定

发布时间：2018-03-05 16:45

本文选题：晕船　切入点：适应　出处：《第二军医大学》2006年博士论文　论文类型：学位论文

【摘要】：目的:研究晕船适应大鼠脑干蛋白质的表达变化,探讨晕船适应的可能机制。方法:根据模拟晕船刺激后大鼠异嗜高岭土量变化建立晕船适应大鼠模型,双向电泳分离鉴定脑干差异表达蛋白质,Western Blot验证差异表达蛋白质谷氨酰胺合成酶,并检测其活性以及脑干谷氨酸、天冬氨酸、γ-氨基丁酸和甘氨酸含量变化。结果:获得了16个晕船适应相关蛋白质,泛素羧基末端水解酶PGP9.5、谷氨酰胺合成酶、苹果酸脱氢酶、硫氧还蛋白过氧化物酶1、硫氧还蛋白过氧化物酶2、血红素加氧酶1、低分子质量神经丝、肝再生相关蛋白、similar to sirtuin和hypothetical protein XP 346711表达上调;M1型丙酮酸激酶、磷酸甘油酸变位酶B、磷酸丙糖异构酶1、线粒体电压依赖型阴离子通道1和碳酸酐酶2表达下调。晕船适应大鼠脑干谷氨酰胺合成酶活力增强,谷氨酸含量升高。结论:大鼠对晕船适应后可以诱导脑干蛋白质的改变,主要与能量代谢、氧化应激和神经递质调节等因素有关。谷氨酸在晕船适应的过程中可能发挥重要作用。
[Abstract]:Objective: to study the changes of brain stem protein expression in seasickness adaptation rats and to explore the possible mechanism of seasickness adaptation. The differential expression protein of brain stem was identified by two dimensional electrophoresis. Western Blot was used to verify the differential expression of glutamine synthase, and its activity and glutamic acid in brain stem were detected. Results: sixteen seasickness related proteins, ubiquitin carboxyl terminal hydrolase PGP 9.5, glutamine synthase, malate dehydrogenase, were obtained. The expression of thioredoxin peroxidase 1, thioredoxin peroxidase 2, heme oxygenase 1, low molecular weight neurofilament, liver regeneration related protein similar to sirtuin and hypothetical protein XP 346711 up-regulated pyruvate kinase M 1. The expression of phosphoglyceric acid translocation enzyme B, propanose phosphate isomerase 1, mitochondrial voltage-dependent anion channel 1 and carbonic anhydrase 2 were down-regulated. Conclusion: the changes of brain stem protein can be induced by seasickness in rats, which is mainly related to energy metabolism. Oxidative stress is related to neurotransmitter regulation. Glutamate may play an important role in seasickness adaptation.
【学位授予单位】：第二军医大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R83

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