持续性高正加速度重复作用导致大鼠损伤的自然修复模型

发布时间：2018-03-09 07:40

本文选题：加速度　切入点：大鼠　出处：《军医进修学院学报》2005年06期 　论文类型：期刊论文

【摘要】：目的:建立持续性高正加速度(+G z)重复作用导致大鼠损伤的自然修复模型。方法:雄性W istar大鼠108只随机分成9组,每组12只。A组为空白对照;B组地面固定5 m in;C组固定于离心机转臂上,俯卧位,头向轴心,+1G z离心5 m in;D组、E组、F组、G组、H组、I组体位同C组,+10G z离心30 s,间隔时+1G z离心60 s,连续5/d,每周4d,共4周。A、B、C、D组于离心次日处死,其余各组分别于离心后1周、2周、4周、8周和12周后次日处死,取颞下颌关节、咀嚼肌、心、肝、肾等器官组织作光镜、电镜观察。结果:①动物全部成活。②A组、B组和C组器官组织未见异常;其余各组除肾脏未见明显异常外,心脏、肝脏和颞下颌关节、咀嚼肌呈现不同程度病理改变,随时间推移,上述损伤逐步减轻、呈现修复改变。结论:本动物模型重复性好,实验证实+G z可以导致心脏、肝脏和颞下颌关节、咀嚼肌损伤,并能自然修复,是研究+G z导致机体损伤和自然修复较理想的动物模型。
[Abstract]:Objective: to establish a natural repair model of injury induced by persistent high positive acceleration (G _ z) in rats. Methods: one hundred and eight male W istar rats were randomly divided into 9 groups. 12 rats in group A. were fixed on the rotating arm of centrifuge in the position of prone position in group B, the ground of group B was fixed at 5 min ~ (-1). Head axis, 1G z centrifugation for 5 min / d group, 1 G z centrifugation for 5 min / d, 1 G z centrifugation for 5 min / d, 4 weeks / week for 4 weeks, 4 weeks / day, respectively, were killed on the second day after centrifugation, and the position of group I was the same as that of group C, 10 G z for 30 s and 1 G z for 60 s, 4 days per week, respectively, and the rats in group C were killed on the second day after centrifugation. The other groups were killed at 1 week, 2 weeks, 4 weeks, 8 weeks and 12 weeks after centrifugation. The temporomandibular joint, masticatory muscle, heart, liver, kidney and other organs were taken for light microscope. Results No abnormality was found in the organs of group B and C, except for the kidney, the heart, liver, temporomandibular joint and masticatory muscles showed pathological changes in different degrees over time. Conclusion: this animal model has good reproducibility. The experiment proved that G z can cause damage to heart, liver, temporomandibular joint, masticatory muscle, and can be repaired naturally. It is an ideal animal model to study the damage caused by G z and natural repair.
【作者单位】：解放军总医院解放军总医院解放军305医院解放军总医院解放军总医院解放军总医院解放军总医院
【基金】：“十五”军队科研基金项目(01Q061)
【分类号】：R85

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