模拟微重力对大鼠睾丸中睾酮合成相关基因表达的影响

发布时间：2018-03-13 18:02

本文选题：模拟微重力　切入点：睾丸　出处：《航天医学与医学工程》2008年06期 　论文类型：期刊论文

【摘要】：目的研究雄性大鼠在模拟微重力下睾丸组织中类固醇激素合成相关基因表达的变化,探讨微重力对睾丸中睾酮合成的影响,以及这种影响与血浆睾酮含量变化的关系。方法建立无隐睾尾部悬吊大鼠的模拟微重力模型;采用酶联免疫竞争(ELISA)法测定大鼠血浆睾酮的含量;逆转录聚合酶链反应(RT-PCR)法检测睾丸组织中类固醇激素合成急性调节蛋白(StAR)、细胞色素P450-17α-羟化酶(P450c17)、3β-羟甾脱氢酶(3β-HSD)的mRNA水平的变化。结果雄性大鼠在模拟微重力条件下7d后,睾丸重量极显著减轻(P0.01),血浆睾酮含量极显著下降(P0.01),睾丸组织中StAR、P450c17、3β-HSD的mRNA水平显著下调(P0.05)。结论模拟微重力引起雄性大鼠睾丸中StAR、P450c17、3β-HSD的表达下调,使睾酮合成途径受阻,导致睾酮分泌减少,进而造成血浆睾酮含量下降。
[Abstract]:Objective to investigate the effects of microgravity on testosterone synthesis in testis of male rats by studying the changes of steroid hormone biosynthesis related gene expression in testis under simulated microgravity. Methods the simulated microgravity model of rats without caudal suspension of cryptorchidism was established and the plasma testosterone level was determined by Elisa. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the changes of mRNA levels of steroid hormone synthesis acute regulatory protein Starn and cytochrome P450-17 伪 -hydroxylase P450 c17 伪 -hydroxylase 3 尾 -hydroxysteroid dehydrogenase 3 尾 -HSD.Results male rats were exposed to simulated microgravity for 7 days. Testicular weight was significantly reduced, plasma testosterone content was significantly decreased, and mRNA level of Starn P450 c173 尾 -HSD was significantly down-regulated in testicular tissue. Conclusion the expression of Starn P450 c173 尾 -HSD in the testis of male rats was down-regulated by simulated microgravity, and the testosterone synthesis pathway was blocked. This leads to a decrease in testosterone secretion, which in turn leads to a decrease in plasma testosterone levels.
【作者单位】：北京理工大学生命科学与技术学院航天生物与医学工程研究所;
【基金】：国家自然科学基金项目(20505002)
【分类号】：R852.22

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