模拟微重力对人脐静脉内皮细胞形态及NO产生的影响

发布时间：2018-03-15 01:36

本文选题：失重模拟　切入点：回转器　出处：《航天医学与医学工程》2005年05期 　论文类型：期刊论文

【摘要】：目的探讨回转模拟微重力对人的血管内皮细胞形态、NO产生及一氧化氮合酶表达的影响。方法利用体外培养的人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC),采用回转器模拟微重力效应,在微重力条件下培养人脐静脉内皮细胞48h,同时设1g静止培养对照。倒置显微镜下观察细胞生长形态,透射电镜观察HUVEC超微结构。检测不同转速下细胞产生NO的量,及细胞中内皮型一氧化氮合酶(eNOS)、诱导型一氧化氮合酶(iNOS)的表达。结果微重力培养后,细胞变圆且重叠生长;回转组NO产生量高于1g对照组且与一定的回转速度呈正相关。回转组细胞eNOS表达较对照组增
[Abstract]:Objective to investigate the effects of rotational simulated microgravity on the production of nitric oxide (no) and the expression of nitric oxide synthase (NOS) in human vascular endothelial cells. Human umbilical vein endothelial cells were cultured under microgravity for 48 h, and 1 g static culture was used as control. The morphology of cells was observed under inverted microscope, the ultrastructure of HUVEC was observed by transmission electron microscope, and the amount of no produced by cells at different rotational speeds was detected. And the expression of endothelial nitric oxide synthase (Enos) and inducible nitric oxide synthase (iNOS). Results after microgravity culture, the cells became round and overlapped. The production of no in the rotary group was higher than that in the control group, and there was a positive correlation between the production of no and the rotation rate. The expression of eNOS in the revolving group was higher than that in the control group.
【作者单位】：哈尔滨医科大学免疫学教研室哈尔滨工业大学生命科学学院哈尔滨医科大学免疫学教研室哈尔滨医科大学免疫学教研室哈尔滨医科大学免疫学教研室哈尔滨工业大学食品科学与遗传工程研究所哈尔滨医科大学免疫学教研室
【基金】：“863”科技项目资助(2003AA744052) 黑龙江省教育厅科技项目资助(10551137)
【分类号】：R85;

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