贫铀污染对伤口愈合的影响及其初步机制研究

发布时间：2018-03-15 11:00

本文选题：贫铀　切入点：创伤　出处：《中国人民解放军军事医学科学院》2004年硕士论文　论文类型：学位论文

【摘要】：目的海湾战争后，贫铀武器的危害及防护研究受到广泛关注。除贫铀弹片伤外，贫铀武器产生的气溶胶也可能污染伤口。本研究对贫铀污染伤口的愈合影响及其相关机理进行探索，旨在为贫铀武器危害及防护研究提供实验依据。方法用手术方法结合贫铀粉尘污染建立贫铀粉尘污染开放性伤口的动物模型以及相应的实验对照组，采用常规病理学，免疫组织化学和图像分析等方法，观察受伤后伤口愈合过程中不同时间点创伤局部炎症细胞，修复细胞，细胞基质，部分生长因子及其受体的表达。结果贫铀污染后将导致创面愈合延迟，且与剂量成正相关关系；贫铀导致机体全身及伤口局部炎症反应增强，肉芽组织生成缓慢，数量减少，成纤维细胞功能低下，胶原纤维合成减少，从而延长伤口愈合时间，降低愈合质量。贫铀刺激伤口局部炎症因子及其受体的合成及分泌，抑制TGF-β、bFGF及其相关受体的表达，可能是贫铀影响伤口愈合的分子机制之一。结论贫铀影响伤口愈合过程中的炎症细胞反应，，修复细胞反应、生长因子及其受体的合成和分泌等关键环节，从而导致愈合时间的延长。
[Abstract]:Purpose. After the Gulf War, the research on the harm and protection of depleted uranium weapons has received extensive attention. In addition to depleted uranium bomb wounds, aerosol produced by depleted uranium weapons may also pollute wounds. This study has explored the effects of wound healing and its related mechanisms on the wound healing caused by depleted uranium. The purpose of this paper is to provide experimental basis for the research on the hazards and protection of depleted uranium weapons. Method. The animal model of the open wound caused by DU dust pollution and the corresponding experimental control group were established by surgical method combined with DU dust pollution. Routine pathology, immunohistochemistry and image analysis were used. The expression of local inflammatory cells, repair cells, cell matrix, partial growth factor and its receptor were observed at different time points during wound healing. Results. The wound healing was delayed after depleted uranium contamination, which was positively correlated with the dose. Depleted uranium resulted in increased inflammation, slow granulation formation, decreased number and low fibroblast function. The synthesis of collagen fibers decreased, thus prolonging wound healing time and reducing healing quality. Depleted uranium stimulated the synthesis and secretion of local inflammatory factors and their receptors, and inhibited the expression of TGF- 尾 bFGF and its related receptors. This may be one of the molecular mechanisms by which depleted uranium affects wound healing. Conclusion. Depleted uranium affects the inflammatory cell response, repair cell response, synthesis and secretion of growth factor and its receptor during wound healing, which leads to the prolongation of healing time.
【学位授予单位】：中国人民解放军军事医学科学院
【学位级别】：硕士
【学位授予年份】：2004
【分类号】：R82

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