回转器模拟失重对大鼠心肌细胞H9c2形态、骨架和增殖能力的影响

发布时间：2018-03-19 01:18

本文选题：失重模拟　切入点：回转器　出处：《航天医学与医学工程》2011年05期 　论文类型：期刊论文

【摘要】：目的探讨模拟失重对大鼠心肌细胞系-H9c2细胞的细胞形态、细胞骨架和增殖能力的影响。方法采用双向多样本回转器(2D-RWVs)模拟失重,在30 r/m in转速下分别培养2,4,6和8 d,用图像处理分析软件(Image J)测量分析模拟失重后细胞面积的改变,激光共聚焦荧光显微镜观察细胞骨架的变化,采用细胞计数法测量细胞生长曲线。结果回转组H9c2细胞在回转培养至8 d比对照组细胞面积显著缩小(P0.001),细胞微丝和微管结构发生改变,改变率为48.48%,而回转培养2,4和6 d与对照组细胞面积、微丝和微管结构无明显差异。细胞生长曲线显示模拟失重8 d时间段,回转组H9c2细胞生长曲线与对照组一致。结论回转器模拟失重8 d导致H9c2细胞面积缩小、细胞微丝解聚以及微管结构紊乱,但其增殖能力未受到影响。
[Abstract]:Objective to investigate the effects of simulated weightlessness on cell morphology, cytoskeleton and proliferation of rat myocardial cell line H9c2.Methods Two-way multi-sample gyroscope (2D-RWVs) was used to simulate weightlessness. The cells were cultured at 30 r / min for 6 and 8 days, respectively. The changes of cell area after simulated weightlessness were measured by image processing analysis software (Image J), and the changes of cytoskeleton were observed by laser confocal fluorescence microscope. The cell growth curve was measured by cell count method. Results the cell area of H9c2 cells in the rotary group was significantly smaller than that in the control group at 8 days, and the cell microfilament and microtubule structure were changed. The change rate was 48.48%, but there was no significant difference in cell area, microfilament and microtubule structure between the control group and the control group for 2 d and 6 d. The cell growth curve showed simulated weightlessness for 8 d. Conclusion the cell area, microfilament depolymerization and microtubule structure of H9c2 cells were reduced after 8 days of simulated weightlessness, but the proliferation ability of H9c2 cells was not affected.
【作者单位】：哈尔滨工业大学生命科学与工程系;哈尔滨工业大学生物医学工程中心;
【基金】：科技部重大研究计划项目资助(2006AA703503E) 国家863计划资助(2008AA12A2)
【分类号】：R852.22

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