原花青素对小鼠氧惊厥的预防作用及其机理

发布时间：2018-03-22 21:00

本文选题：氧惊厥　切入点：原花青素　出处：《第二军医大学》2005年硕士论文　论文类型：学位论文

【摘要】：急性氧中毒是当机体暴露在氧压大于200 kPa(2 ATA)的环境中时,可能发生的一种疾病,主要表现为中枢神经系统的症状,类似于癫痫大发作。近年来,随着高压氧治疗在临床上日益广泛的应用,以及人们在进军海洋过程中潜水活动逐渐增多,接触高压氧的机会也越来越多。由于人体的氧耐受性、仪器设备、操作方法等因素,发生急性氧中毒的可能性有所增加,所以做好急性氧中毒的预防工作十分必要。药物预防是一种必要的辅助方式。虽然也对一些药物进了研究,但是由于某些药物在预防效果上存在问题,或者是其在使用过程中所带来的毒副作用令人困扰,因此到目前为止,尚没有找到较好的预防药物。我们的实验对原花青素(procyanidins,PC)的预防作用进行了观察。结合急性氧中毒致病机理的研究,提出了PC的预防作用主要是与它的抗氧化特性和对脑血管的保护作用有关的假设。围绕这一假设,我们观察了PC对高压氧暴露导致小鼠惊厥潜伏期、惊厥大发作时间和脱离高压氧环境恢复期的影响,为了研究其具体的预防机制,我们同时测定了接受高压氧暴露后的小鼠脑组织中活性氧(reactive oxygen species,ROS)、丙二醛(malonaldehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)等与机体氧化-抗氧化系统有关指标的含量变化以及脑内重要的抑制性神经递质γ-氨基丁酸(γ-GABA)的含量变化。研究结果显示,PC可以明显延长5个大气压的纯氧暴露下小鼠氧惊厥的潜伏期,同时可明显减少经上述处理的小鼠皮层和海马中ROS、MDA的含量,增加SOD的含量,以及增加脑组织中GABA的含量。通过研究我们认为,PC具有肯定的预防氧惊厥的作用,这种预防作用可能与以下两个因素有关:一是它有较强的氧自由基清除活性,从而增强机体的抗氧化能力;二是通过抗氧化作用,间接调节脑内的神经递质的平衡及稳定脑组织内环境等作用对脑组织产生保护作用。
[Abstract]:Acute oxygen poisoning is a disease that may occur when the body is exposed to an oxygen pressure greater than 200 kPa(2 ATAs, mainly manifested as symptoms of the central nervous system, similar to seizures in recent years. With the increasing clinical application of hyperbaric oxygen therapy, and the increasing number of diving activities in the process of entering the ocean, the chances of exposure to hyperbaric oxygen are also increasing. The possibility of acute oxygen poisoning has increased, so it is necessary to do a good job in the prevention of acute oxygen poisoning. Drug prevention is a necessary auxiliary method. Although some drugs have also been studied, However, because some drugs have some problems in the preventive effect, or because of the harmful side effects in the course of their use, so far, no good preventive drugs have been found. The preventive effect of procyanidins PCS was observed in our experiment. The prophylaxis of PC is mainly related to its antioxidant properties and its protective effect on cerebral vessels. Around this hypothesis, we observed the latent period of convulsion induced by hyperbaric oxygen exposure in mice. The effect of convulsion time and recovery from hyperbaric oxygen environment, in order to study its specific prevention mechanism, We also measured the contents of reactive oxygen species (oxygen), malondialdehyde (MDA) and superoxide dismutase (SOD) in the brain tissues of mice exposed to hyperbaric oxygen (HBO), and the changes of the contents of the indexes related to the oxidation-antioxidant system and the important inhibition in the brain. Changes of 纬-aminobutyric acid (纬 -GABA), a neurotransmitter. The results showed that PC could significantly prolong the incubation period of oxygen convulsion in mice exposed to pure oxygen at 5 atmospheres, at the same time, it could significantly reduce the content of SOD in cortex and hippocampus of mice treated above, and increase the content of SOD. And increase the content of GABA in brain tissue. Through the study, we think that PC can prevent oxygen convulsion, this preventive effect may be related to the following two factors: first, it has strong oxygen free radical scavenging activity, thereby enhancing the antioxidant capacity of the body; The other is to indirectly regulate the balance of neurotransmitters in brain and stabilize the environment of brain tissue through antioxidation.
【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R82

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