多坝式空间细胞培养室设计参数的微重力实验验证

发布时间：2018-03-27 08:40

本文选题：空间细胞培养室　切入点：微重力　出处：《航天医学与医学工程》2010年04期

【摘要】：目的利用失重飞行提供的微重力环境验证多坝式空间细胞培养室的设计参数。方法设计了三坝式和五坝式两种结构的插片细胞培养室,在失重飞机抛物线飞行中,通过研究培养室内流场形态、液流更换效率及流速对室内细胞的影响,对其在微重力下的适用性进行了验证。结果三坝或五坝式结构均能在室内隔离形成有效液流通路,确保微重力环境下培养室内溶液的有效更替;插片式设计在方便取出细胞样品用于后续分析的同时,可将细胞培养片固定在培养室底部防止刮蹭细胞;微重力下50μL/min和150μL/min两种换液流速均不会损伤细胞。结论综合考虑空间细胞培养的高容积利用率及短操作时间等需求,插片式三坝细胞培养室结构及150μL/min的换液流速更符合应用要求。
[Abstract]:Objective to verify the design parameters of multi-dam space cell culture chamber by microgravity environment provided by weightlessness flight. The effects of flow field morphology, fluid exchange efficiency and flow velocity on the indoor cells were studied and its applicability under microgravity was verified. Results the effective liquid flow path could be formed in the indoor isolation of the three-dam or five-dam structures. To ensure the effective replacement of the solution in the culture room in microgravity environment, the insert design can fix the cell culture piece at the bottom of the culture room to prevent the cell from scrape while the cell sample can be taken out for subsequent analysis. Under microgravity, both 50 渭 L/min and 150 渭 L/min fluid exchange velocity could not injure the cells. Conclusion considering the demand of high volume utilization rate and short operation time of space cell culture, the structure of intercalated Sanba cell culture chamber and the liquid exchange flow rate of 150 渭 L/min are more suitable for application.
【作者单位】：中国航天员科研训练中心航天医学基础与应用国家重点实验室;
【基金】：国家重点基础研究发展计划(2006CB705704) 中国航天医学工程预先研究项目(2008SY5414004)
【分类号】：R854

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