回转模拟失重对静脉内皮细胞血管生成能力的影响及内皮型一氧化氮合成酶的作用

发布时间：2018-03-27 12:46

本文选题：模拟失重　切入点：回转器　出处：《航天医学与医学工程》2013年02期

【摘要】：目的观察回转器模拟失重对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC-C)血管生成能力的影响,并分析探讨可能涉及的关键信号分子内皮型一氧化氮合成酶(endothelial nitricoxide synthetase,eNOS)的作用。方法体外培养HUVEC-C,随机分为回转模拟失重组、1 G静止对照组及回转+抑制剂组,选用内皮型一氧化氮合成酶的特异性抑制剂L-NAME(N-nitro-L-arginine methyl es-ter hydrochloride)。Matrigel胶小管形成实验观察不同处理组HUVEC-C血管生成能力的变化情况。RT-PCR和Western blot分别检测模拟失重前、后HUVEC-C中eNOS mRNA和蛋白的表达变化。结果24 h模拟失重使HUVEC-C的血管生成能力较对照组明显增强(P0.01),且这种增强效应可以被L-NAME所抑制。模拟失重24 h后,HUVEC-C中eNOS mRNA和蛋白的表达均显著高于对照组(P0.05)。结论回转模拟失重可以诱导HUVEC-C血管生成能力增强,其发生机制与eNOS表达上调有关。
[Abstract]:Objective to observe the effect of simulated weightlessness on the angiogenesis of human umbilical vein endothelial cells (HUVEC-C) in human umbilical vein. The effect of endothelial nitric oxide synthase synthesizer (nitricoxide) on endothelial nitric oxide synthase (nitricoxide) was analyzed. The changes of angiogenesis ability of HUVEC-C in different treatment groups were observed by using L-NAME(N-nitro-L-arginine methyl es-ter hydrochloride).Matrigel, a specific inhibitor of endothelial nitric oxide synthase. RT-PCR and Western blot were used to detect the changes of HUVEC-C angiogenesis ability before simulated weightlessness. Results after 24 h simulated weightlessness, the angiogenesis ability of HUVEC-C was significantly enhanced than that of control group, and this enhancement effect could be inhibited by L-NAME. After 24 h simulated weightlessness, eNOS mRNA and protein in HUVEC-C were inhibited. Conclusion rotational simulated weightlessness can induce HUVEC-C angiogenesis. Its mechanism is related to the upregulation of eNOS expression.
【作者单位】：第四军医大学航空航天医学系航空航天生物动力学教研室航空航天医学教育部重点实验室;
【基金】：国家自然科学基金(81171872,30900279) 陕西省自然科学(2010JQ4016)
【分类号】：R852.22

【相似文献】