模拟微重力条件下奥金肽对MC3T3-E1前成骨细胞增殖的影响

发布时间：2018-03-30 20:06

本文选题：模拟微重力　切入点：奥金肽　出处：《军事医学》2014年11期

【摘要】：目的研究模拟微重力(simulated microgravity,SMG)条件下奥金肽(osgentide,OST)对小鼠前成骨细胞MC3T3-E1增殖功能的影响。方法在正常细胞培养环境下,利用MTT法检测6种OST化合物对MC3T3-E1细胞增殖的影响,从中筛选出有效作用浓度的化合物,进一步利用MTT实验及流式细胞术分别检测SMG条件下1 nmol/L OST5对MC3T3-E1细胞的增殖效应及细胞周期的分布。结果在正常细胞培养环境下,1 nmol/L OST5对MC3T3-E1细胞的增殖具有显著促进作用(P0.01)。MTT实验结果表明,与正常细胞培养环境相比,MC3T3-E1细胞在SMG条件下其增殖受到显著抑制。在SMG条件下,用1 nmol/L OST5处理MC3T3-E1细胞(OST-SMG)3d,流式细胞术检测结果表明,SMG促使更多的MC3T3-E1细胞进入G1期。1 nmol/L OST5处理MC3T3-E1细胞后S期比例比SMG条件下显著提高(P0.05),表明OST5能够促进DNA合成。结论在SMG条件下,OST5能够促进成骨细胞的增殖,为研究OST5对模拟微重力相关的骨丢失防治提供了理论依据。
[Abstract]:Objective to study the effect of osgentide OST on the proliferation of mouse preosteoblasts (MC3T3-E1) under simulated microgravity simulated microgravity (SMG).Methods MTT assay was used to detect the effect of six OST compounds on the proliferation of MC3T3-E1 cells.MTT assay and flow cytometry were used to detect the proliferation effect and cell cycle distribution of 1 nmol/L OST5 on MC3T3-E1 cells under SMG condition.Results the proliferation of MC3T3-E1 cells was significantly promoted by 1 nmol/L OST5 in normal cell culture. The results showed that the proliferation of MC3T3-E1 cells was significantly inhibited under SMG compared with that in normal cells culture environment.Under the condition of SMG, MC3T3-E1 cells were treated with 1 nmol/L OST5 for 3 d. The results of flow cytometry showed that SMG could induce more MC3T3-E1 cells to enter G1 phase of nmol/L OST5 and increase the proportion of S phase of MC3T3-E1 cells compared with SMG, which indicated that OST5 could promote DNA synthesis.Conclusion OST5 can promote the proliferation of osteoblasts under the condition of SMG, which provides a theoretical basis for the study of the prevention and treatment of bone loss related to simulated microgravity by OST5.
【作者单位】：解放军第306医院特种医学实验研究中心;中国医学科学院北京协和医学院药物研究所;中国科学院理化技术研究所光化学转换与功能材料重点实验室;
【分类号】：R855

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