模拟微重力对大鼠嗜铬细胞瘤细胞形态及其生长的影响

发布时间：2018-04-02 15:11

本文选题：失重模拟　切入点：水平式回转器　出处：《航天医学与医学工程》2006年02期

【摘要】：目的研究回转是否影响PC12细胞内蛋白质的羰基化和硝基化水平,进而影响细胞骨架和形态;细胞内活性氮水平升高是否会影响PC12细胞的增殖分化。方法观察并测定了静置对照组和回转组细胞的细胞形态和细胞密度的变化,采用免疫荧光标记和RT-PCR技术研究了回转对细胞骨架蛋白Ac-tin羰基化和Tubulin硝基化以及nNOS和iNOS的mRNA和蛋白表达的影响;并利用流式细胞术检测了细胞周期的改变,初步探讨了这些改变和一氧化氮合酶之间的关系。结果细胞骨架蛋白发生硝基化和羰基化的改变,但回转组细胞骨架形态变化不明显,PC12细胞的微丝回转后明显伸长;回转后,细胞密度显著增加,细胞周期中DNA合成的S期明显缩短。结论结果表明水平回转模拟微重力并不改变PC12细胞形态,相反还有利于细胞的生长,其机制可能与一氧化氮合酶的表达上调有关。
[Abstract]:Objective to study whether gyration affects the carbonylation and nitration of proteins in PC12 cells, thus affecting the cytoskeleton and morphology, and whether the increase of intracellular active nitrogen level will affect the proliferation and differentiation of PC12 cells.Methods the changes of cell morphology and cell density in static control group and rotary group were observed and measured.The effects of rotation on the carbonylation and Tubulin nitration of cytoskeleton protein Ac-tin and the expression of mRNA and protein of nNOS and iNOS were studied by immunofluorescence labeling and RT-PCR technique, and the changes of cell cycle were detected by flow cytometry.The relationship between these changes and nitric oxide synthase (NOS) was discussed.Results the cytoskeleton proteins were changed by nitration and carbonylation, but the cytoskeleton morphology of the cells in the rotary group was not obviously changed.The S phase of DNA synthesis was significantly shortened in cell cycle.Conclusion the results suggest that the horizontal gyration simulated microgravity does not change the morphology of PC12 cells, but it is also beneficial to the growth of PC12 cells. The mechanism may be related to the up-regulation of nitric oxide synthase (NOS) expression.
【作者单位】：中国航天员科研训练中心中国航天员科研训练中心中国航天员科研训练中心中国航天员科研训练中心中国航天员科研训练中心
【基金】：中国载人航天项目基金资助
【分类号】：R85

【相似文献】