模拟微重力对NIH-3T3细胞clock及bmal1基因表达的影响

发布时间：2018-04-08 21:55

本文选题：模拟微重力　切入点：近日节律　出处：《航天医学与医学工程》2012年01期

【摘要】：目的探讨模拟微重力对NIH-3T3细胞节律基因clock与bmal1 mRNA表达的影响。方法 NIH-3T3细胞采用回转器分别回转培养6 h,12 h,18 h,24 h,30 h,36 h,42 h和48 h,同时设置1 G静置培养为对照组。Trizol试剂提取细胞总RNA,实时定量PCR法检测节律基因clock及bmal1 mRNA在各时间点的相对表达量。通过余弦法获取节律参数,并经振幅检验分析是否存在昼夜节律。结果 clock及bmal1基因mRNA的表达趋势相近。对照组clock与bmal1 mRNA相对水平呈现节律性表达,而回转组表达无节律性。回转组mRNA相对水平最大值出现于约6 h时间点,最小值出现在约18 h,而对照组分别出现于约18 h与24 h。与对照组比回转组的周期延长了约16 h。回转后clock与bmal1的峰值相位前移。结论 NIH-3T3细胞中clock与bmal1的表达存在同步化的转录特征。模拟微重力可影响clock与bmal1节律的周期长度及峰值相位。
[Abstract]:Objective to investigate the effect of simulated microgravity on the expression of rhythm gene clock and bmal1 mRNA in NIH-3T3 cells.Methods NIH-3T3 cells were cultured in gyrator for 6 h, 12 h, 18 h, 24 h, 30 h, 36 h, 42 h and 48 h, respectively. At the same time, 1 G static culture was used as the control group. Trizol reagent was used to extract total RNAs. Real-time quantitative PCR assay was used to detect the rhythmic gene clock and bmal1 mRNA at each time.The relative expression of an intermediate point.The rhythm parameters are obtained by cosine method, and the circadian rhythm is analyzed by amplitude test.Results the trend of mRNA expression of clock and bmal1 genes was similar.The expression of clock and bmal1 mRNA in the control group was rhythmic, while that in the gyration group was not rhythmic.The relative level of mRNA appeared at about 6 h, the minimum at about 18 h, and the control group at about 18 h and 24 h, respectively.Compared with the control group, the cycle of the gyration group was prolonged by about 16 hours.The peak phase of clock and bmal1 moves forward after rotation.Conclusion the expression of clock and bmal1 in NIH-3T3 cells is characterized by synchro transcription.Simulated microgravity can affect the cycle length and peak phase of clock and bmal1 rhythms.
【作者单位】：燕山大学环境与化学工程学院;中国航天员科研训练中心航天医学基础与应用国家重点实验室;航天城门诊部;四川大学华西基础医学与法医学院;
【基金】：航天医学基础与应用国家重点实验室自主项目(SMFA09A06) 中国载人航天预先研究项目(SJ200908) 国家重点基础研究计划资助项目(2011CB711000)
【分类号】：R852.6

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