太空环境对甘草生理生化的影响

发布时间：2018-04-11 04:06

本文选题：甘草 + 航天　；参考：《中国中药杂志》2010年02期

【摘要】：目的:探讨太空环境对甘草生理生化诱变作用分析。方法:利用返回式卫星搭载甘草,18 d后返回地球(飞行回收舱平均辐射剂量为0.102 m.d-1,飞行远地点距地球350 km,重力为1×10-6m.s-2),搭载种子返回地面后与对照组同时种植于实验田中,对太空搭载后甘草的过氧化氢酶(CAT),超氧化物歧化酶(SOD)的活性,甘草的蛋白含量以及蛋白电泳进行了考察。结果:甘草经过卫星搭载后,甘草CAT,SOD酶活性均有不同程度提高,差异明显(P0.05),而且鄂托克前旗比杭锦旗酶活性均高。甘草经过卫星搭载后,甘草蛋白含量有一定程度提高,差异明显(P0.05),同时甘草蛋白电泳也产生了差异,产生了微弱的新的条带。结论:太空环境对甘草蛋白类产生一定影响,这些变化对于后期选育甘草优良种质奠定基础。
[Abstract]:Objective: to study the effect of space environment on physiological and biochemical mutagenesis of Glycyrrhiza uralensis.Methods: the recoverable satellite was used to carry Glycyrrhiza uralensis back to Earth for 18 days (the average radiation dose of the flight recovery module was 0.102 m.d-1, the apogee was 350km from the Earth, the gravity was 1 脳 10-6 m.s-2.The seeds were returned to the ground and planted in the experimental field with the control group at the same time.The activity of catalase (CAT), superoxide dismutase (SOD), protein content and protein electrophoresis of Glycyrrhiza uralensis were studied.Results: after carrying Glycyrrhiza uralensis by satellite, the SOD activity of Glycyrrhiza uralensis CATO was increased to some extent, the difference was obvious (P0.05), and the enzyme activity of Qianqi flag was higher than that of Hangjin Banner.The content of Glycyrrhiza uralensis protein increased to a certain extent after carried by satellite, and the difference was obvious (P0.05). At the same time, the electrophoresis of Glycyrrhiza uralensis protein also produced the difference, resulting in a weak new band.Conclusion: space environment has some effect on glycyrrhiza protein, and these changes lay a foundation for later breeding of fine liquorice germplasm.
【作者单位】：天津大学药物科学与技术学院;天津科技大学中药生物工程研究所;中国药材集团公司;
【基金】：国家自然科学基金项目(30472148,30772732)
【分类号】：R85

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