驻京某部队新兵结核潜伏感染人群筛查新方法研究

发布时间：2018-04-12 08:42

本文选题：结核潜伏感染 + CFP-ESAT融合蛋白　；参考：《军事医学》2017年06期

【摘要】：目的了解2014年驻京某部队入伍新兵结核感染情况,同时对一种新的结核潜伏感染筛查方法进行评价。方法 194名入伍新兵,行胸部X线检查、结核菌素试验(PPD)、抗体检测,同时应用ELISA联合重组融合蛋白CFP10-ESAT6和潜伏感染蛋白Rv2628进行IGRA检测。结果 PPD皮肤试验和抗体检测的阳性率分别为49.7%和15.5%。CFP10-ESAT6刺激后γ干扰素释放试验(IGRA)检测发现194名新兵中潜伏感染率为22.2%。Rv2628刺激结核潜伏感染(latent tuberculosis infection,LTBI)人群后产生IFN-γ的水平显著高于健康对照(P0.05),其刺激PPD弱阳性组(5 mm≤皮试直径15 mm)产生的IFN-γ值显著高于强阳性组(皮试直径≥15 mm)(P0.05),而CFP10-ESAT6刺激后产生的IFN-γ水平在这两组间差异不显著(P0.05)。CFP10-ESAT6和Rv2628刺激后产生的IFN-γ水平在抗体阴、阳性组间无显著性差异(P0.05)。Rv2628诊断结核感染的ROC曲线下面积为0.84,约登指数为0.621,此时特异度为94.7%,敏感度为67.4%。结论联合检测抗原Rv2628及CFP10-ESAT6特异的IFN-γ值在鉴别结核活动或潜伏感染方面具有一定潜能和价值。
[Abstract]:Objective to investigate tuberculosis infection in recruits in Beijing in 2014 and to evaluate a new screening method for latent tuberculosis infection.Methods 194 recruits were examined by chest X-ray, tuberculin test and antibody, and IGRA was detected by ELISA combined with recombinant fusion protein CFP10-ESAT6 and latent infection protein Rv2628.Results the positive rates of PPD skin test and antibody test were 49.7% and 49.7%, respectively. The detection of interferon 纬 release test after 15.5%.CFP10-ESAT6 stimulation showed that the latent infection rate in 194 recruits was 22.2%.Rv2628 stimulated tuberculosis latent infection latent tuberculosis infection (LTBI) population and produced IFN- 纬.And IFN- 纬 produced by Rv2628 stimulation in antibody negative,There was no significant difference between the positive groups. The area under the ROC curve was 0.84, the Yorden index was 0.621, the specificity was 94.7 and the sensitivity was 67.4.Conclusion the combined detection of Rv2628 and CFP10-ESAT6 specific IFN- 纬 values has a certain potential and value in differentiating tuberculosis activity or latent infection.
【作者单位】：解放军316医院检验科;解放军309医院全军结核病研究所全军结核病防治重点实验室结核病诊疗新技术北京市重点实验室;
【基金】：总参军事医学和老年病科研基金资助项目(ZCWS14C03)
【分类号】：R825.2

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