谷胱甘肽转硫酶M1和T1基因型与高原反应的危险性

发布时间：2018-04-15 02:21

本文选题：高海拔 + 谷胱甘肽转移酶　；参考：《中华劳动卫生职业病杂志》2005年03期

【摘要】：目的探讨谷胱甘肽转硫酶M1、T1基因型(GSTM1、GSTT1)与高原反应危险性的关系。方法从同一生活和工作环境中选取123名男性武警战士作为调查对象,根据是否发生急性高原反应,将其分成病例组和对照组,其中病例组43人、对照组80人。基因组DNA来自研究对象提供的外周血有核细胞,采用多重聚合酶链反应(PCR)方法对这些武警战士的谷胱甘肽转硫酶M1和T1基因进行分型。结果病例组GSTT1非缺失型基因频率为69.8%,明显高于正常对照组(42.5%),差异有统计学意义(P=0.004,OR=3.12,95%CI为1.42～6.86)。两组GSTM1缺失型基因频率分别为72.1%和52.5%,差异有统计学意义(P=0.03,OR=2.34,95%CI为1.05～5.02)。GSTT1阴性GSTM1阴性基因型者发生高原反应的危险性比携带GSTT1阴性GSTM1阳性者高5倍(OR=5.04;95%CI为1.00～25.3)。结论谷胱甘肽转硫酶M1、T1基因多态性与高原反应危险性有关。
[Abstract]:Objective to investigate the relationship between glutathione transferase (GSTM1) and risk of high altitude reaction (GSTT1).Methods 123 male armed police soldiers were selected from the same living and working environment. According to the occurrence of acute altitude reaction, they were divided into two groups: the case group (43 cases) and the control group (80 cases).Genomic DNA was derived from peripheral blood nucleated cells provided by the study subjects. The glutathione transferase M1 and T1 genes of these armed police soldiers were genotyped by multiplex polymerase chain reaction (PCR).Results the frequency of GSTT1 non-deletion gene in the case group was 69.8, which was significantly higher than that in the normal control group (42.5%). The difference was statistically significant (3.1295 CI = 1.42 卤6.86).The frequency of GSTM1 deletion gene in the two groups was 72.1% and 52.5, respectively. The difference was statistically significant (P < 0.05). The risk of high altitude reaction was 5 times higher in the patients with negative 1.05~5.02).GSTT1 GSTM1 genotype than in those with GSTT1 negative GSTM1 positive genotype.Conclusion the polymorphism of glutathione transferase M 1 T 1 gene is associated with the risk of high altitude reaction.
【作者单位】：华中科技大学同济医学院职业医学研究所武警四川总队成都医院检验科华中科技大学同济医学院职业医学研究所武警四川总队成都医院检验科武警四川总队成都医院检验科武警四川总队成都医院检验科武警四川总队成都医院检验科武警四川总队成都医院检验科华中科技大学同济医学院职业医学研究所
【基金】：国家自然科学基金资助项目(30371204) 成都武警医院研究基金资助项目
【分类号】：R82

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