间断性水平位站立对尾吊大鼠胫骨骨小梁结构影响的micro-CT评价

发布时间：2018-04-28 09:54

本文选题：尾吊大鼠 + 间断性水平位站立　；参考：《中国骨质疏松杂志》2013年05期

【摘要】：目的应用micro-CT研究尾吊大鼠胫骨骨小梁微结构的变化,以此评价间断性水平位站立对抗模拟失重导致的骨微结构退化的效果。方法 24只SD雄性大鼠随机等分为3组:对照组(CON组)、尾吊组(SUS组)、水平位站立对抗组(ST组)。尾吊组大鼠始终保持约30°头低位及后肢自由悬垂不负重状态,水平位站立对抗组在尾吊处理的基础上,大鼠每天固定时间水平站立1 h。实验3周后处死各组大鼠,取其右侧胫骨行micro CT扫描及三维重建,对其骨微结构进行定量分析。结果选定感兴趣区(ROI)行定量分析及三维可视化后,与CON组相比,SUS组胫骨骨体积分数(BVF)、骨小梁数量(Tb.N)、骨小梁厚度(Tb.Th)均显著降低,而骨表面积/体积(BS/BV)、骨小梁间隙(Tb.Sp)及骨小梁模式因子(Trabecular Pattern Factor,TPF)均显著升高;ST组的变化介于SUS组和CON组之间;三维可视化图像结果与物理参数结果趋势一致。应用One-Way ANOVA分析及多重比较LSD-t检验,三组各参数之间统计学差异显著(P0.05)。结论间断性水平位站立可明显减弱尾吊大鼠胫骨的微结构退化和骨质丢失,而micro CT可以有效地辨别胫骨松质骨微结构的精细变化。
[Abstract]:Objective to study the changes of trabecular microstructures in tibial bone of tail suspended rats by micro-CT, and to evaluate the effect of intermittent horizontal standing on bone microstructure degeneration induced by simulated weightlessness. Methods 24 Sprague-Dawley male rats were randomly divided into three groups: control group (Con group), tail suspension group (SUS group), horizontal standing antagonism group (St group). The rats in the tail suspension group maintained a 30 掳head-low position and a free-hanging hind limb without weight bearing. The rats in the horizontal position standing antagonism group stood at a fixed time for 1 hour per day on the basis of tail suspension treatment. After 3 weeks the rats were killed and their right tibia were taken for micro CT scan and 3D reconstruction. The bone microstructure was quantitatively analyzed. Results after quantitative analysis and 3D visualization in selected area of interest (ROI), compared with CON group, the tibial bone volume fraction, the number of trabecular bone and the thickness of trabecular bone were significantly decreased in SUS group compared with CON group. However, the changes of bone surface area / volume, trabecular gap Tb.Spand trabecular Pattern factorial factor in St group were significantly higher than those in SUS group and CON group, and the results of 3D visualization were consistent with those of physical parameters. Using One-Way ANOVA analysis and multiple comparison LSD-t test, the statistical differences among the three groups were significant (P 0.05). Conclusion intermittent horizontal standing can significantly attenuate the microstructural degeneration and bone loss of tibia in tail-suspended rats, and micro CT can effectively distinguish fine changes of cancellous bone microstructure in tibia.
【作者单位】：第四军医大学航空航天生物动力学教研室航空航天医学教育部重点实验室;第四军医大学口腔医院显微CT实验室;
【基金】：国家自然科学基金项目(31170889,30870595)
【分类号】：R852.22

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