模拟失重对不同发育阶段大鼠骨代谢的影响

发布时间：2018-05-09 19:56

本文选题：失重 + 骨胳　；参考：《第一军医大学学报》2002年07期

【摘要】：目的　比较模拟失重对不同发育阶段大鼠骨代谢和负重骨生物力学性能影响的差异。方法　对不同月龄SD大鼠尾吊4周后,以生化和放免法测动物骨代谢指标;生物材料试验机测负重骨生物力学性能;灰化法和原子吸收分光光度法测骨矿盐和钙量,并进行比较。结果　（1）尾吊4周,大鼠骨形成指标均显著下降（P0.01）;（2）尾吊鼠血清钙显著增加（P0.01）;（3）除弹性负荷外（P0.05）,尾吊使负重骨各力学指标均显著下降（P0.01）,且最大桡度和最大负荷下降与月龄有关（P0.05）;（4）尾吊对不同月龄鼠胫骨体积、湿重减少的影响不同（P0.05）;（5）年幼鼠骨矿盐含量减少4.32％,高于成年鼠的2.78％,但钙丢失为0.53％,远低于成年鼠的3.05％。结论　尾吊模拟失重对不同发育阶段大鼠骨代谢和负重骨生物力学性能变化的影响程度不同。
[Abstract]:Objective to compare the effects of simulated weightlessness on bone metabolism and biomechanical properties of loaded bone in rats at different developmental stages. Methods the bone metabolism indexes were measured by biochemical and radioimmunoassay, biomechanical properties of loaded bone were measured by biomaterial testing machine, bone mineral salt and calcium content were measured by ashing and atomic absorption spectrophotometry after 4 weeks of tail suspension in SD rats of different months of age. And the comparison was made. Results 1) tail suspension for 4 weeks. Rat bone formation index decreased significantly (P 0.01) serum calcium increased significantly in tail suspension rats (P 0.01) except elastic load, all mechanical parameters of loaded bone decreased significantly (P 0.01), and the maximum radial and maximum load decreased with age related to age (P 0.05 ~ (4) tail suspension. Tibia volume of rats of the same age, The content of bone mineral salt in young rats decreased by 4.32, which was higher than that in adult rats by 2.78, but the calcium loss was 0.53, which was much lower than that of adult rats (3.05). Conclusion the effects of simulated weightlessness of tail suspension on bone metabolism and biomechanical properties of loaded bone in rats at different developmental stages are different.
【作者单位】：第一军医大学南方医院创伤骨科第一军医大学南方医院创伤骨科第一军医大学南方医院检验科第一军医大学南方医院检验科
【基金】：广东省自然科学基金（980226）
【分类号】：R852.22

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