模拟失重对成骨细胞整合素亚单位表达的影响

发布时间：2018-05-11 05:14

本文选题：失重 + 成骨细胞　；参考：《第四军医大学学报》2006年12期

【摘要】：目的:观察回转器模拟失重对成骨细胞整合素亚单位表达的影响.方法:培养的大鼠乳鼠颅骨成骨随机分为对照组和失重组(用回转器模拟失重条件),在实验的24,48和72h提取细胞总RNA,进行反转录PCR,检测整合素的α5,αv和β1亚单位mRNA表达,并计算与内参照β肌动蛋白(beta-actin)mRNA水平的比值.同时用Westernblotting检测各种整合素亚单位的蛋白表达变化.结果:从模拟失重24h开始,整合素亚单位的基因表达即发生改变,但在对照组中,基因表达无明显变化.整合素α5mRNA在模拟失重的24,48和72h分别下降了11.3%,18.7%和9.8%;整合素αvmR-NA分别下降
[Abstract]:Aim: to observe the effect of simulated weightlessness on the expression of integrin subunit in osteoblasts. Methods: calvarial osteogenesis of cultured neonatal rats was randomly divided into control group and weightlessness group. Total RNAs were extracted at 24 h and 72 h after weightlessness. The expression of 伪 5, 伪 v and 尾 1 subunits of integrin was detected by reverse transcription of PCRs. The ratio of 尾 -actin to beta-actinine mRNA was calculated. At the same time, Westernblotting was used to detect the protein expression of various integrin subunits. Results: from 24 h after simulated weightlessness, the gene expression of integrin subunit changed, but in the control group, the gene expression did not change significantly. Integrin 伪 5mRNA decreased by 11.3% and 9.8% at 24 h and 72 h after simulated weightlessness, and integrin 伪 vmR-NA decreased by 18.7% and 9.8%, respectively, and integrin 伪 vmR-NA decreased respectively at 24 h and 72 h after simulated weightlessness.
【作者单位】：第四军医大学航空航天医学系航空航天生物动力学教研室第四军医大学航空航天医学系航空航天生物动力学教研室中国航天员科研训练中心中国航天员科研训练中心第四军医大学航空航天医学系航空航天生物动力学教研室第四军医大学航空航天医学系航空航天生物动力学教研室
【基金】：总装备部重点试验技术项目(2001301)
【分类号】：R852.22

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