正加速度重复暴露后大鼠心室肌缝隙连接蛋白43表达及分布的改变

发布时间：2018-05-13 00:26

本文选题：加速度 + 心肌　；参考：《生理学报》2008年03期

【摘要】：本文旨在探讨不同水平的一周重复多次正加速度(+Gz)暴露后大鼠心室肌缝隙连接蛋白43(connexin 43,Cx43)表达及分布的改变。36只雄性Sprague-Dawley大鼠随机分为对照组、+6Gz组和+10Gz组,每组12只。+Gz组大鼠分别暴露于+6Gz和+10Gz各3min,1次/d,共1周,分别于末次暴露后即刻、1d、3d、7d(各3只)取心室肌作免疫组织化学染色和Western blot分析,检测Cx43的表达和分布。免疫组织化学结果显示,+Gz重复暴露可引起大鼠心室肌Cx43分布方式明显紊乱,Cx43在心肌细胞侧-侧连接处的表达明显增加,在心肌细胞端-端连接处的表达减少。Western Blot结果显示,+6Gz组与+10Gz组的Cx43蛋白表达量于末次暴露后即刻、1d、3d、7d与对照组相应时刻相比均明显减少(P0.001),以暴露后即刻减少最为明显,且随着+Gz暴露时间的延长,表达逐渐恢复,但在暴露后7d,两组的Cx43表达量仍未恢复至对照组水平。Cx43蛋白表达的上述改变在+10Gz组比+6Gz组更为显著。以上结果提示+Gz重复暴露可引起大鼠心室肌Cx43表达量的一过性减少,分布方式明显紊乱,这种改变很可能是+Gz致心律失常发生的重要原因之一。
[Abstract]:The purpose of this study was to investigate the expression and distribution of gap junction protein (43(connexin 43) in ventricular muscle of rats exposed to repeated positive acceleration (GZ) at different levels. 36 male Sprague-Dawley rats were randomly divided into control group, 6Gz group and 10Gz group with 12 in each group. The rats in Gz group were exposed to 6Gz and 10Gz for 3 mins / d for 1 week, respectively. The ventricular muscles were taken from each group for 7 days (3 rats) immediately after the last exposure. The expression and distribution of Cx43 were detected by immunohistochemical staining and Western blot analysis. The results of immunohistochemistry showed that repeated exposure to Gz could significantly increase the expression of Cx43 in the lateral and lateral junctions of rat ventricular myocytes. The results of Western Blot showed that the expression of Cx43 protein in the 6Gz group and the 10Gz group was significantly decreased at 1 day after the last exposure and 3 days after the last exposure, compared with the corresponding time in the control group. The most obvious decrease was at the moment after exposure, compared with the corresponding time of the control group, the results showed that the expression of Cx43 protein in the 6Gz group and the 10Gz group was significantly decreased at 7 days after the last exposure, compared with that in the control group. With the prolongation of Gz exposure time, the expression of Cx43 recovered gradually, but at 7 days after Gz exposure, the expression of Cx43 protein did not return to the level of control group. The above changes in the expression of Cx43 protein were more significant in 10Gz group than in 6Gz group. These results suggest that repeated exposure to Gz may cause a transient decrease in the expression of Cx43 in rat ventricular muscles and a significant disorder in the distribution pattern, which may be one of the important causes of arrhythmias induced by Gz.
【作者单位】：第四军医大学航空航天医学系航空航天生物动力学教研室航空航天医学教育部重点实验室;
【基金】：the Elitist Foundation of Chinese People’s Liberation Army (No. 04J012) Special PurposeTopic of Chinese People’s Liberation Army (No. 06Z010)
【分类号】：R852.21

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