大鼠腹部开放伤合并海水浸泡后内毒素、TNF-α、IL-6、IL-8的变化

发布时间：2018-05-14 12:00

本文选题：海水 + 内毒素　；参考：《军事医学科学院院刊》2009年05期

【摘要】：目的:观察大鼠腹部开放伤合并海水浸泡后不同时段血浆内毒素(LPS)、肿瘤坏死因子-α(TNF-α)、IL-6和IL-8的变化。方法:成年健康Wistar大鼠116只,随机分为2组,其中腹部开放伤合并海水浸泡组96只,腹部开放伤对照组20只。应用微生物快速检测仪和γ测量仪检测大鼠腹部开放伤合并海水浸泡后0.5,1,2,3和4h血浆LPS、TNF-α、IL-6和IL-8水平。结果:腹部开放伤合并海水浸泡2～4h后,血浆中LPS水平明显上升,与对照组比较具有显著性差异。海水浸泡4h后血浆中TNF-α浓度上升明显,具有显著性差异。海水浸泡3～4h后血浆中IL-6浓度明显升高,具有显著性差异。海水浸泡后不同时间血浆中IL-8浓度与对照组比较无明显变化。结论:血浆中LPS、TNF-α和IL-6浓度明显升高与损伤关系密切,是引发中毒性休克导致机体死亡的主要原因之一。
[Abstract]:Objective: to observe the changes of plasma endotoxin (LPS), tumor necrosis factor- 伪 (TNF- 伪), IL-6 and IL-8 in rats with open abdominal injury combined with seawater immersion in different periods. Methods: 116 healthy adult Wistar rats were randomly divided into two groups: 96 rats with open abdominal injury and seawater immersion group, and 20 rats with open abdominal injury group. The plasma levels of TNF- 伪 IL-6 and IL-8 in rats with abdominal open wound and seawater immersion were measured by microbiological rapid detection instrument and 纬 -meter. Results: after 24 hours of seawater immersion, the level of LPS in the plasma was significantly higher than that in the control group. After being immersed in seawater for 4 h, the concentration of TNF- 伪 in plasma increased obviously, and there was a significant difference. The concentration of IL-6 in plasma was significantly increased after seawater immersion for 34 h. There was no significant change in plasma IL-8 concentration at different time after seawater immersion compared with control group. Conclusion: the increase of plasma LPSN TNF- 伪 and IL-6 levels is closely related to the injury and is one of the main causes of death caused by toxic shock.
【作者单位】：海军总医院海战伤救治研究中心;
【基金】：海军医学科研重点课题资助项目(04HW13)
【分类号】：R82

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