细胞凋亡在腹部开放伤合并海水浸泡大鼠肠屏障功能障碍中的作用

发布时间：2018-05-17 12:40

本文选题：腹部损伤 + 海水浸泡　；参考：《实用医学杂志》2008年21期

【摘要】：目的:探讨肠黏膜上皮细胞的凋亡在大鼠腹部开放伤合并海水浸泡后肠屏障功能障碍中的作用。方法:观察腹腔海水浸泡后血浆二胺氧化酶(DAO)、内毒素(LPS)含量变化,肠组织丙二醛(MDA)、超氧化物歧化酶(SOD)含量,肠黏膜组织病理损伤程度;利用原位末端标记法计数肠黏膜上皮细胞凋亡指数。结果:腹部开放伤合并海水浸泡后,大鼠血浆DAO、LPS水平显著升高;肠组织MDA含量显著升高,SOD活性显著降低(P0.05或P0.01);小肠病理组织学检查显示肠黏膜屏障受损;小肠黏膜上皮细胞凋亡指数显著增加(P0.01)。结论:腹部开放伤合并海水浸泡可导致大鼠肠屏障功能障碍,出现肠源性内毒素血症,肠组织氧自由基损伤,肠黏膜上皮细胞凋亡明显增加,上皮细胞凋亡增加在肠屏障功能障碍发生中可能起重要作用。
[Abstract]:Aim: to investigate the role of intestinal epithelial cell apoptosis in intestinal barrier dysfunction after abdominal open injury and seawater immersion in rats. Methods: the contents of plasma diamine oxidase (DAOX), endotoxin (LPS), malondialdehyde (MDA), superoxide dismutase (SOD) in intestinal tissue and the degree of pathological injury in intestinal mucosa were observed after seawater immersion in abdominal cavity. Apoptosis index of intestinal mucosal epithelial cells was counted by in situ end labeling method. Results: after abdominal open injury combined with seawater immersion, the plasma Dao-LPS level was significantly increased, the intestinal MDA content was significantly increased and the activity of sod was significantly decreased (P0.05 or P0.01), the intestinal mucosal barrier was damaged by histopathological examination. Apoptosis index of intestinal mucosal epithelial cells increased significantly (P 0.01). Conclusion: open abdominal injury combined with seawater immersion can lead to intestinal barrier dysfunction, intestinal endotoxemia, intestinal tissue oxygen free radical injury and intestinal mucosal epithelial cell apoptosis. Increased apoptosis of epithelial cells may play an important role in the development of intestinal barrier dysfunction.
【作者单位】：第二军医大学附属长征医院普通外科;北京海军总医院普通外科;海战伤救治研究中心;
【分类号】：R83

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