噪声性听觉损伤易感豚鼠的差异表达基因筛选研究

发布时间：2018-05-29 09:28

本文选题：噪声性听觉损伤 + 易感性　；参考：《第二军医大学》2007年硕士论文

【摘要】： 噪声性听觉损伤(NIHL)是军事噪声环境下官兵常发生的一种听力疾患。本研究观察了不同噪声暴露量对豚鼠听觉功能和听觉器官结构的影响,探讨了NIHL易感性研究的最佳噪声暴露方案,建立了NIHL易感豚鼠模型,利用抑制性消减杂交技术筛选NIHL易感与不易感豚鼠耳蜗的差异表达基因片段148条,生物信息学结果显示同源性大于80%且匹配碱基大于100 bp的片段共34条。初步分析提示线粒体基因组和热休克蛋白与NIHL及其易感性的发生相关。研究结果将为进一步探讨NIHL易感性的发生机制提供基础。
[Abstract]:Noise-induced hearing impairment (NIHL) is a common hearing disorder in military noise environment. In this study, the effects of different noise exposures on auditory function and auditory organ structure in guinea pigs were observed, the optimal noise exposure scheme for the study of NIHL susceptibility was discussed, and the NIHL susceptible guinea pig model was established. Suppression subtractive hybridization was used to screen 148 differentially expressed genes from the cochlea of susceptible and unsusceptible guinea pigs. Bioinformatics showed that there were 34 fragments with homology of more than 80% and matching base of more than 100bp. Preliminary analysis indicated that mitochondrial genome and heat shock protein were associated with NIHL and its susceptibility. The results will provide a basis for further study on the pathogenesis of NIHL susceptibility.
【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R82

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