高分压氧处理对体外培养大鼠脾淋巴细胞增殖及杀伤能力的影响

发布时间：2018-06-02 00:16

本文选题：高分压氧 + 脾淋巴细胞　；参考：《中国职业医学》2006年04期

【摘要】：目的观察大鼠体外脾淋巴细胞经不同压力—时程的高分压氧处理后淋巴细胞增殖及细胞毒性T淋巴细胞(CTL)杀伤能力的变化。方法分离成年大鼠脾淋巴细胞,置于高压细胞培养舱内,以不同压力—时程的高分压氧(含CO25 kPa)处理,W ST-8比色法测定经刀豆球蛋白(ConA)刺激后淋巴细胞的增殖率和对肿瘤细胞株P815的杀伤能力。以常压空气(含5%CO2)处理作为对照组。结果一定压力—时程的高分压氧处理对体外培养的大鼠脾淋巴细胞增殖及杀伤能力具有促进作用,但随暴露剂量的增加则逐渐表现为抑制效应。结论高分压氧对离体大鼠脾淋巴细胞增殖及杀伤能力存在一个从适度暴露促进功能发挥至过量暴露抑制功能发挥的过程。
[Abstract]:Objective to observe the changes of lymphocyte proliferation and cytotoxic T lymphocyte cytotoxicity (CTL) of splenic lymphocytes treated with high pressure and high pressure oxygen in vitro. Methods the spleen lymphocytes of adult rats were isolated and placed in the culture chamber of high pressure cells. The proliferation rate of lymphocytes stimulated by concanavalin (Cona) and its cytotoxicity to tumor cell line P815 were determined by high pressure oxygen (including CO25 KPA) treatment with W ST-8 colorimetry. The control group was treated with atmospheric air (including 5CO _ 2). Results the hyperbaric oxygen treatment with a certain pressure-time course promoted the proliferation and killing ability of rat splenic lymphocytes in vitro, but gradually showed inhibitory effect with the increase of exposure dose. Conclusion there is a process from moderate exposure to excessive exposure to the proliferation and cytotoxicity of splenic lymphocytes in vitro by high partial pressure oxygen.
【作者单位】：中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室第二军医大学海军医学系潜水医学教研室第二军医大学海军医学系潜水医学教研室中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室第二军医大学海军医学系潜水医学教研室
【基金】：国家自然科学基金资助项目(30400159)
【分类号】：R84

【参考文献】