短波紫外线照射四肢火器伤后组织中羟脯氨酸含量的变化

发布时间：2018-06-08 05:28

本文选题：紫外线 + 火器　；参考：《中国组织工程研究与临床康复》2007年01期

【摘要】：目的:观察四肢火器伤受不同剂量短波紫外线照射后,组织中羟脯氨酸含量的变化,以期分析紫外线照射对胶原蛋白合成的影响。方法:实验于2003-09/2004-09在解放军总医院理疗科实验室和解放军军事医学科学院放射医学研究所完成。选用新西兰大白兔90只,随机数字法分为3组:30mJ/cm2照射组、60mJ/cm2照射组和对照组,每组30只。麻醉动物后,以“五四”式手枪击兔右股部肌肉丰满处,造成贯通伤模型。常规清创后采用紫外线治疗仪分别对3组实验动物伤道进行30s(30mJ/cm2)照射,60s(60mJ/cm2)照射和无照射干预。在致伤后第2和3天继续在伤道内进行照射,日增加前次照射剂量的30%。采用化学法在伤后7,14,21,28,42,56和70d测量肉芽组织中羟脯氨酸含量的变化。结果:实验动物82只进入结果分析。①致伤后7d,各组大白兔组织中羟脯氨酸的含量无差别。②伤后14d,60mJ/cm2照射组羟脯氨酸的含量高于30mJ/cm2照射组和对照组[(11.91±0.49)mg/g,(10.88±0.61)mg/g,(10.35±0.85)mg/g,P0.05];伤后21～56d,60mJ/cm2照射组伤口中的羟脯氨酸含量均高于30mJ/cm2照射组伤口和未照射组(P0.01)。③伤后70d时,各组羟脯氨酸的含量下降接近正常组织水平,60mJ/cm2照射组仍稍高于30mJ/cm2照射组和未照射组[(10.02±0.65)mg/g,(9.85±0.54)mg/g,(9.27±0.43)mg/g,P0.05]。④在伤后21～56d30mJ/cm2照射组伤口中羟脯氨酸的含量高于对照组(P0.05)。结论:短波紫外线照射能促进伤口中羟脯氨酸的合成,增加胶原含量,60mJ/cm2照射的作用强于30mJ/cm2。
[Abstract]:Aim: to observe the changes of hydroxyproline content in the tissues after different doses of ultraviolet radiation on firearm injury in extremities, and to analyze the effect of ultraviolet irradiation on collagen synthesis. Methods: the experiment was carried out in the Laboratory of Physiotherapy Department of PLA General Hospital and the Institute of Radiology of PLA Academy of military Medical Sciences between September 2003 and September 2004. Ninety New Zealand white rabbits were randomly divided into 3 groups: 30 mg / cm 2 and 60 mg / cm 2 in each group. After anaesthesia, the "May fourth" pistol was used to strike the muscle of the right thigh of the rabbit to make a penetrating injury model. After conventional debridement, three groups of experimental animals were treated with ultraviolet ray therapy instrument for 30 s ~ 30 mg / cm ~ (2) irradiation and 60 mg / cm ~ (2) irradiation respectively. On the 2nd and 3rd day after injury, the irradiation was continued in the wound tract, and the dose of the previous irradiation was increased by 30 times per day. The changes of hydroxyproline content in granulation tissue were measured by chemical method on day 7, 14, 21, 28, 4256 and 70 days after injury. Results: at 7 days after injury, there was no difference in the content of hydroxyproline in rabbits. 2. The content of hydroxyproline in 60 mg / cm 2 group was higher than that in 30 mg / cm 2 group and control group (11.91 卤0.49 mg / g / g group 10.88 卤0.61 mg / g / kg group, 10.35 卤0.85 mg / cm ~ (-1) P 0.05), and the content of hydroxyproline was 10.35 卤0.85 mg / g / cm ~ (5) at 21 ~ 56d ~ 60 mg / cm ~ (2) after injury in the experimental animals (11.91 卤0.49 mg / g / g) group and control group (P < 0.05). The content of hydroxyproline in wound of group A was higher than that of group B (30 mg / cm 2) and that of group B (P 0.01C 3) 70 days after injury. The content of hydroxyproline in 60 mg / cm2 irradiation group was still slightly higher than that in 30 mg / cm 2 irradiation group and no irradiation group [10.02 卤0.65 mg / g] .4 the hydroxyproline content was 9.27 卤0.43 mg / g / g P 0.05 at 21 56 days after injury and was higher than that in control group P0.055.The content of hydroxyproline in the injury group was higher than that in the control group (P 0.05). Conclusion: short wave ultraviolet irradiation can promote the synthesis of hydroxyproline in the wound and increase the collagen content of 60 mg / cm 2 than that of 30 mg / cm 2.
【作者单位】：解放军总医院理疗科解放军总医院理疗科解放军总医院理疗科
【基金】：军队“十五”青年基金项目资助(01YQ17)~~
【分类号】：R826

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