晕船及适应过程中大鼠血浆及脑氨基酸含量变化

发布时间：2018-06-08 08:17

本文选题：晕船 + 氨基酸　；参考：《营养学报》2006年06期

【摘要】：目的:通过观察模拟晕船及适应后大鼠血浆及脑内游离氨基酸含量变化,为进一步研究防治晕船的营养干预措施提供实验依据。方法:采用Crampton模型模拟晕船刺激,以异嗜高岭土行为作为判定大鼠晕船及晕船适应的指标,运用高效液相色谱法测定晕船及晕船适应过程中大鼠血浆及脑内游离氨基酸含量变化。结果:模拟晕船刺激1d后,血浆胱氨酸和异亮氨酸含量显著升高,Gly、Pro含量显著下降,BCAA/AAA的比值升高;脑内Ala、Cys+Met、Tyr、His以及总氨基酸含量显著下降;模拟晕船刺激21d后血浆及脑内游离氨基酸无明显变化,脑内Glu/GABA显著升高。晕船耐受与晕船易感大鼠血浆及脑内游离氨基酸含量、血浆BCAA/AAA比值及脑内Glu/GABA比值均无显著差异。结论:晕船刺激使实验大鼠血浆和脑内氨基酸含量发生明显改变,其中有些与神经递质合成有关的氨基酸改变较显著。
[Abstract]:Objective: to observe the changes of free amino acids in plasma and brain after simulated seasickness and adaptation in order to provide experimental evidence for further study on nutritional intervention measures for prevention and treatment of seasickness. Methods: Crampton model was used to simulate seasickness and isophilic kaolin behavior was used as an index to judge seasickness and seasickness adaptation in rats. The contents of free amino acids in plasma and brain during seasickness and seasickness adaptation were measured by high performance liquid chromatography (HPLC). Results: after stimulated by simulated seasickness for 1 day, the contents of plasma cystine and isoleucine increased significantly and the ratio of BCAA / AAA increased significantly, and the contents of Ala-Cys Meta-TyrHis and total amino acids in the brain decreased significantly. After simulated seasickness stimulation for 21 days, there were no significant changes in plasma and brain free amino acids, but Glu-P / GABA was significantly increased in the brain. There was no significant difference in plasma and brain free amino acids, plasma BCAA / AAA ratio and brain Glu-P / GABA ratio between seasickness tolerance and seasickness susceptible rats. Conclusion: the contents of amino acids in plasma and brain of experimental rats were significantly changed by seasickness stimulation, some of which were related to neurotransmitter synthesis.
【作者单位】：第二军医大学海医系军队卫生教研室第二军医大学海医系军队卫生教研室第二军医大学海医系军队卫生教研室
【分类号】：R835

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