钙蛋白酶介导模拟失重大鼠心肌肌钙蛋白抑制亚基的降解

发布时间：2018-06-08 20:04

  本文选题：钙蛋白酶 + 钙蛋白酶抑素　； 参考：《生理学报》2010年05期

【摘要】：本文旨在观察尾部悬吊模拟失重大鼠心肌钙蛋白酶(calpain)与钙蛋白酶抑素(calpastatin)表达的变化,以探讨心肌肌钙蛋白抑制亚基(cardiac troponin I,cTnI)降解的可能机制。采用尾部悬吊模拟失重大鼠模型,Western blotting技术观测心肌calpain-1、calpain-2与calpastatin的表达;PD150606抑制calpain活性,分析cTnI降解程度的变化。结果显示:与同步对照组相比,悬吊2周与4周组大鼠心肌calpastatin表达呈显著性降低(P0.05),calpain-1表达未改变,calpain-2表达略有降低;但是,心肌calpain-1/calpastatin及calpain-2/calpastatin的比值在悬吊2周与4周组明显增高(P0.05,P0.01)。悬吊4周组cTnI降解显著高于对照组(P0.01);然而,用calpain非特异性抑制剂PD150606处理后,对照组及悬吊组cTnI的降解均被显著抑制(P0.01)。这些结果提示模拟失重大鼠心肌calpain活性增高可能增加cTnI的降解。
[Abstract]:The aim of this study was to investigate the changes of cardiac calpainin and calpastatin expression in tail suspended simulated weightlessness rats, in order to explore the possible mechanism of cardiac troponin inhibiting the degradation of cardiac troponin IcTnI. Western blotting technique was used to observe the expression of calpain-1 calpain-2 and calpastatin in rat model of simulated weightlessness by tail suspension. PD150606 inhibited the activity of calpain and analyzed the degradation degree of cTnI. The results showed that compared with the control group, the expression of calpastatin in rat myocardium was significantly decreased after 2 and 4 weeks of suspension, but the ratio of calpain-1/calpastatin to calpain-2/calpastatin was significantly increased in the groups of 2 weeks and 4 weeks of suspension, but the expression of calpain-2 was not changed slightly, however, the ratio of calpain-1/calpastatin to calpain-2/calpastatin was significantly increased in the groups of 2 weeks and 4 weeks of suspension. The degradation of cTnI in the suspension group was significantly higher than that in the control group (P 0.01), however, the degradation of cTnI in both the control group and the suspension group was significantly inhibited after treatment with PD150606, a non-specific inhibitor of calpain. These results suggest that the increase of myocardial calpain activity may increase the degradation of cTnI in simulated weightlessness rats.
【作者单位】： 第四军医大学航空航天生理教研室;
【基金】：supported by the National Natural Science Foundation of China(No.30770805)
【分类号】：R85
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本文编号：1997025