尾吊对大鼠离体肺循环的影响及NOS抑制剂的对抗作用

发布时间：2018-06-10 06:47

本文选题：模拟失重 + 尾吊　；参考：《航天医学与医学工程》2011年04期

【摘要】：目的研究尾吊模拟失重对大鼠离体肺循环的影响和NOS抑制剂的对抗作用,为模拟失重对肺循环局部调节和立位耐力和运动能力降低机制研究积累资料。方法 -30°尾吊(TS)模拟失重的生理效应,Wistar雄性大鼠被随机分成3组:对照组(Con,n=8)、7 d尾吊组(TS7,n=10)和14 d尾吊组(TS14,n=11)。在人工胸腔内,采用离体肺灌流技术,超声流量计和恒流蠕动泵将肺循环流量控制在13.58 mL/min。PowerLab/4SP生理记录系统检测模拟失重对大鼠最大肺动脉压、静脉压和阻力影响和10-5 mol/L L-NAME的对抗作用。结果 TS7,TS14组大鼠离体灌流的肺动脉压最大值显著降低(P0.01),肺动脉端阻力和肺循环阻力显著降低(P0.01),10-5 mol/L L-NAME能较好对抗模拟失重对肺循环的影响。结论 NOS抑制剂对抗立位耐力和运动能力降低有应用前景。
[Abstract]:Objective to study the effects of tail suspension simulated weightlessness on isolated pulmonary circulation and the antagonistic effect of NOS inhibitor on pulmonary circulation in rats, and to study the mechanism of simulated weightlessness on the local regulation of pulmonary circulation and the decrease of standing endurance and exercise ability. Methods male Wistar rats were randomly divided into three groups: control group (control group), control group (control group) for 7 days, and tail suspension group for 14 days, and rats in tail suspension group for 14 days were divided into three groups: the control group (control group) and the control group (control group), and the control group was divided into three groups: the control group (control group) and the 14-day tail suspension group (n = 11). In the artificial thoracic cavity, in vitro lung perfusion technique, ultrasonic Flowmeter and constant flow peristalsis pump were used to control the pulmonary circulation flow at 13.58 mL / min. PowerLabP / 4SP physiological record system was used to detect the maximal pulmonary artery pressure in rats after simulated weightlessness. The influence of venous pressure and resistance and the antagonism of 10-5 mol / L L-name. Results in TS7 / TS14 group, the maximal pulmonary artery pressure and pulmonary vascular resistance were significantly decreased, and the pulmonary artery end resistance and pulmonary vascular resistance were significantly decreased. 10-5 mol / L L-NAME could antagonize the effects of simulated weightlessness on pulmonary circulation. Conclusion NOS inhibitors may be used to antagonize orthostatic endurance and decrease of exercise ability.
【作者单位】：中国航天员科研训练中心航天医学基础与应用国家重点实验室;
【基金】：中国载人航天工程基金资助
【分类号】：R852.22

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