模拟失重对MG-63细胞形态、增殖及周期的影响

发布时间：2018-06-16 04:34

本文选题：失重模拟 + 细胞面积　；参考：《航天医学与医学工程》2007年06期

【摘要】：目的探讨模拟失重对人骨肉瘤成骨样细胞(MG-63)细胞面积、增殖、周期的影响。方法采用回转器模拟失重,用图像处理分析软件(Image J)测量分析模拟失重后细胞面积的改变,用细胞计数法测定细胞的生长曲线,用流式细胞仪测定细胞周期。结果回转器模拟失重后,回转组MG-63细胞24h、36h和48h时间段的细胞面积较对照组缩小(P0.05),回转组24h、36h、48h3个时间段与12h时间段比较细胞面积缩小(P0.05),而对照组各时间段之间无明显差异。细胞生长曲线显示模拟失重后细胞增殖明显受抑制。12h、36h、48h回转组MG-63细胞的细胞周期与对照组相比无明显差异,24h回转后G0+G1期细胞显著减少(P0.05),S期细胞则显著增多(P0.05)。结论回转器模拟失重使MG-63细胞在24h、36h和48h细胞面积缩小,12h、24h、36h和48h增殖生长受到抑制,24h时出现细胞周期阻滞。
[Abstract]:Objective to investigate the effects of simulated weightlessness on the area, proliferation and cycle of osteosarcoma like cells (MG-63). Methods the weightlessness was simulated by gyrator, the cell area after simulated weightlessness was measured and analyzed by image processing analysis software (Image J), the cell growth curve was measured by cell count method, and cell cycle was measured by flow cytometry. Results after simulated weightlessness, the cell area of MG-63 cells in the rotary group was smaller than that in the control group at 24 h, 36 h and 48 h, while in the control group, the cell area was smaller than that in the control group at 24 h, 36 h, 48 h and 12 h, respectively. The cell growth curve showed that the cell cycle of MG-63 cells was significantly inhibited after simulated weightlessness. The cell cycle of MG-63 cells in 48 h gyration group was not significantly different from that in control group. Conclusion the cell cycle arrest of MG-63 cells was observed at 24 h, 36 h and 48 h after simulated weightlessness by gyrator, and cell cycle arrest occurred at 24 h, 24 h, 36 h and 48 h after the proliferation and growth of MG-63 cells were inhibited.
【作者单位】：第四军医大学航空航天医学系航空航天生物动力学教研室第四军医大学航空航天医学系航空航天生物动力学教研室第四军医大学航空航天医学系航空航天生物动力学教研室第四军医大学航空航天医学系航空航天生物动力学教研室第四军医大学航空航天医学系航空航天生物动力学教研室第四军医大学航空航天医学系航空航天生物动力学教研室
【基金】：军队十一五专项课题(06Z042)
【分类号】：R85

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