不同预适应对海水浸泡束缚大鼠应激性溃疡的影响

发布时间：2018-06-21 12:29

本文选题：应激性溃疡 + 动物模型　；参考：《第二军医大学学报》2008年10期

【摘要】：目的:建立束缚及海水浸泡致应激性溃疡的动物模型,探讨预适应对应激性溃疡的作用机制及不同应激时间对溃疡影响。方法:Wistar雄性大鼠60只均分成6组:A组为正常对照组,B组为直接应激组,C组为预适应Ⅰ组,D组为预适应Ⅱ组,E组为预适应Ⅲ组,F组为雷尼替丁组。C、D、E组预适应时间分别为0.5h/d、1h/d、1h/d连续5d,F组雷尼替丁灌胃,每日2ml/100g,连续6d。应激:B、C、D、F组海水浸泡8h,E组海水浸泡10h。采用放射免疫法测定应激性溃疡大鼠血清内皮素和6-酮-前列腺素F1α水平变化,通过Guth法评定溃疡指数,溃疡局部胃组织行病理组织学检查。结果:C组、D组及F组血清内皮素水平显著低于B组(P0.01,P0.05,P0.01);C组、D组及F组血清6-酮-前列腺素F1α水平显著高于B组(P0.01,P0.05,P0.01);C组、D组及E组溃疡指数显著低于B组(P0.01,P0.05,P0.01);C组、D组及F组病理较B组改善。E组与B组各指标无明显差别。结论:预适应可有效减轻大鼠应激性溃疡,但应激时间太长可抵消预适应的保护作用。
[Abstract]:Objective: to establish an animal model of stressful ulceration induced by shackles and seawater immersion, and to explore the mechanism of preconditioning stress ulcer and the effect of different stress time on ulceration. Methods: 60 Wistar male rats were divided into 6 groups: group A as normal control group, group B as direct stress group, group C as preconditioning I, group D as preconditioning group II, group E preconditioning The pre adaptation time of group F was.C, D, and group E was 0.5h/d, 1h/d, 1h/d continuous 5D, F group of ranitidine gavage, daily 2ml/100g, and 6D. stress: B, seawater immersion and seawater immersion were used to determine the levels of serum endothelin and prostaglandin alpha in stress ulcer rats by radioimmunoassay. The serum endothelin level in group C, D group and F group was significantly lower than that of group B (P0.01, P0.05, P0.01) in group D and group F, and the level of the serum 6- ketone prostaglandin alpha in the D group and F group was significantly higher than that in the B group. 1); in group C, group D and group F, there was no significant difference between group B and.E in group B. Conclusion: preconditioning could effectively alleviate stress ulcers in rats, but the time of stress was too long to counteract the protective effect of preconditioning.
【作者单位】：解放军第454医院消化科;中国药科大学生理学教研室;解放军第454医院肿瘤科;
【分类号】：R835.2

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