空气潜水减压病大鼠模型的建立

发布时间：2018-06-28 04:46

本文选题：减压病 + 动物模型　；参考：《环境与健康杂志》2009年11期

【摘要】：为建立空气潜水减压病大鼠模型,选用成年清洁级SD大鼠,以适当"压力—时程—减压速率"高气压暴露方案处理,分组如下:(1)600kPa-90min暴露-200kPa/min减压(n=8);(2)700kPa-90min暴露-200kPa/min减压(n=8);(3)700kPa-100min暴露-100kPa/min减压(n=8);(4)700kPa-100min暴露-200kPa/min减压(n=37)。出舱后置于转笼中以3m/min速度运动30min。从整体行为学、大体及显微病理学、炎症反应等方面筛选建立评估指标体系。通过控制高气压暴露的"压力—时程—减压速率"以及转笼运动方法,能制备出具有稳定发病率的大鼠减压病模型,并能很好地观测。建立了包括行为学、肺和中枢神经系统大体病理和显微病理以及炎性指标在内的模型评估体系。能根据需要建立特定发病率的减压病大鼠模型,建立的评估体系能很好地反映模型的病情。
[Abstract]:In order to establish a rat model of air diving decompression sickness, adult clean SD rats were selected and treated with appropriate pressure - time - decompression rate hyperbaric exposure program. The following groups were as follows: (1) 600kPa-90min exposure -200kPa/min decompression (n=8); (2) 700kPa-90min exposure -200kPa/ min decompression (n=8); (3) 700kPa-100min exposure -100kPa/min decompression (n=8); (4) 700kPa-1 00min exposes -200kPa/min decompression (n=37). After being out of the cabin, it is placed in a cage with 3m/min speed movement 30min. from the overall behavior, gross and micropathology, and the inflammatory response is selected to establish the evaluation index system. By controlling the "pressure time - pressure reduction rate" and the method of rotation cage movement, a stable disease can be prepared. A model evaluation system including behavior, general pathology, micropathology, and inflammatory markers in the lung and central nervous system was established. The model of decompression sickness in a specific incidence can be established according to the need. The established evaluation system can reflect the condition of the model well.
【作者单位】：中国人民解放军第二军医大学海军医学系潜水医学教研室;
【基金】：军队“十一五”科技攻关项目(08G064)
【分类号】：R-332;R84

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