航天大鼠模型胸腺蛋白质组学的研究

发布时间：2018-06-29 07:01

本文选题：应急噪音 + 尾吊　；参考：《中国协和医科大学》2007年硕士论文

【摘要】： 目的建立航天大鼠(应急噪音和尾吊)模型，模拟航空应急噪音和微重力环境，研究在此类应激刺激下大鼠胸腺蛋白质变化水平，探索大鼠免疫机能与应急噪音暴露及尾吊之间的联系，推断太空微重力和返回地面时的应急噪音暴露对航天人员免疫系统的影响。方法SD大鼠24只，随机平均分为3组：尾吊组、应急噪音刺激组和正常对照组。对大鼠加以不同刺激饲养，建立模型。处死大鼠后提取胸腺蛋白，应用双向凝胶电泳技术及基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)进行分离、鉴定，通过MASCOT软件(版本2.2)在NCBI nr数据库中检索蛋白质信息。建立大鼠胸腺蛋白质表达谱，并从中筛选各组间差异表达的蛋白质。结果应用安玛西亚imagemaster 5.0软件分析得到每组8个样品的共同蛋白质胶点数分别为对照组214个、尾吊组218个、噪音组256个；共得到航天大鼠模型胸腺蛋白质表达谱中201个蛋白质，其中应急噪音暴露组与正常对照组差异表达蛋白有36个，其中上调24个，下调12个。主要执行新陈代谢、信号转导、酶、信号调节、细胞骨架、免疫、神经传导和离子通道等生物学功能，其中与细胞骨架和新陈代谢相关蛋白质的变化最为明显；正常对照组与尾吊组差异蛋白为37个，其中24个蛋白表达上调，13个蛋白表达下降，其功能涉及新陈代谢、信号转导、黏附、转录和凋亡等。总之，，航天飞行环境对免疫功能和胸腺萎缩方面的影响符合最初的预测。结论本研究表明蛋白质组学技术可以用于研究航天飞行过程中宇航员的某些生理变化。
[Abstract]:Objective to establish a model of spaceflight rats (emergency noise and tail suspension) to simulate the airborne emergency noise and microgravity environment, and to study the changes of thymus protein levels in rats stimulated by such stress. To explore the relationship between immune function and emergency noise exposure and tail suspension in rats, the effects of space microgravity and emergency noise exposure on spaceflight personnel's immune system were inferred. Methods Twenty-four SD rats were randomly divided into three groups: tail suspension group, emergency noise stimulation group and normal control group. Rats were fed with different stimuli to establish a model. Thymus protein was extracted from rats after being killed. The protein was isolated and identified by two-dimensional gel electrophoresis and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). The protein information was retrieved from NCBI nr database by MASCOT software (version 2.2). The rat thymus protein expression profile was established and the differentially expressed proteins were screened. Results the common protein gel number of 8 samples in each group were 214 in control group, 218 in tail suspension group and 256 in noise group by imagemaster 5.0 software, and 201 proteins in thymus protein expression profile of space rat model were obtained. There were 36 differentially expressed proteins between the emergency noise exposure group and the normal control group, of which 24 were up-regulated and 12 were down-regulated. It mainly performs biological functions such as metabolism, signal transduction, enzyme, signal regulation, cytoskeleton, immunity, nerve conduction and ion channel, among which the changes of cytoskeleton and metabolism-related proteins are most obvious. There were 37 differentially expressed proteins in normal control group and tail suspension group, among which 24 proteins were up-regulated and 13 proteins were down-regulated. Their functions were related to metabolism, signal transduction, adhesion, transcription and apoptosis. Overall, the effects of the space flight environment on immune function and thymus atrophy were consistent with initial predictions. Conclusion this study suggests that proteomics can be used to study some physiological changes of astronauts during space flight.
【学位授予单位】：中国协和医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R85

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