模拟失重对大鼠心肌组织细胞因子基因表达谱的影响

发布时间：2018-07-06 12:11

本文选题：失重模拟 + 心肌组织　；参考：《航天医学与医学工程》2008年01期

【摘要】：目的研究模拟失重对大鼠心肌组织中部分细胞因子基因表达谱的影响,探讨模拟失重条件下心脏结构/功能可能发生的变化机制。方法将成年雄性Wistar大鼠随机分为正常对照组(Con)及尾部悬吊模拟失重组(SUS),应用包含96种基因的细胞因子基因芯片检测各组细胞因子表达水平,选取部分表达变化细胞基因,应用Realtime-PCR给予验证。结果与Con组相比,模拟失重可导致心肌组织中17种细胞因子基因表达差异超出2倍,5种(IFNA4、IL-15、IL-1b、LT-b、FGF7)被上调,12种(IGF-1、IGF-II、FGF5、VEGF-D、VEGF-C、IFNr、IL-11、IL-12B、IL-13、IL-17、IL-18、CD40L)被下调。其中,生长因子呈现较普遍下调趋势,炎性类细胞因子表达水平变化不一。结论模拟失重2wk可导致心肌组织中细胞因子网络调控平衡偏移,促生长性、保护性细胞因子普遍下调,而致病性、炎性细胞因子部分明显上调,其可能是失重/模拟失重条件下心肌组织功能结构重塑的重要调控机制。
[Abstract]:Objective to study the effect of simulated weightlessness on the gene expression profile of some cytokines in rat myocardium and to explore the possible mechanism of changes in cardiac structure / function under simulated weightlessness. Methods Adult male Wistar rats were randomly divided into normal control group (Con) and tail suspended simulated weightlessness group (SUS). Cytokine gene chip containing 96 genes was used to detect cytokine expression level in each group. Realtime-PCR was used to verify the results. Results compared with Con group, simulated weightlessness resulted in down-regulated expression of 17 cytokine genes (IFNA4, IL-15, IL-1b, LT-bFGF7) (IGF-1, IGF-IIF, FGF5FGF5, VEGF-DEGF-DEGF-DEGF-11, IL-12, IL-13, IL-17, IL-18, CD40L). The expression of inflammatory cytokines varied with the down-regulation of growth factors. Conclusion simulated weightlessness 2wk can induce the balance shift of cytokine network regulation, promote growth, down-regulate protective cytokines, and up-regulate pathogenicity and inflammatory cytokines in myocardium. It may be an important regulatory mechanism of myocardial functional structure remodeling under weightlessness / simulated weightlessness.
【作者单位】：西北工业大学生命科学院中国航天员科研训练中心中国航天员科研训练中心中国航天员科研训练中心西安交通大学医学院中国航天员科研训练中心中国航天员科研训练中心中国航天员科研训练中心西安交通大学医学院
【基金】：国家自然科学基金资助(30400158)
【分类号】：R852.22

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