不同强度的模拟推拉动作致大鼠海马神经元损伤的实验研究

发布时间：2018-07-10 05:39

本文选题：海马 + 神经元　；参考：《航天医学与医学工程》2004年02期

【摘要】：目的探讨不同强度的推拉动作所致的推拉效应对大鼠海马神经元损伤的程度和时相性。方法应用动物模型评分、病理、电镜技术分别对不同强度模拟推拉动作后大鼠海马各区神经元损伤情况进行检测。结果 1 )动物模型评分 :随着推拉动作强度的加大 ,卒中指数和神经症状评分逐渐增高。 2 )HE染色 :对照组和± 2Gz暴露后各组及± 6Gz、± 8Gz暴露后的 30min组海马各区神经元形态及分布未见异常。± 6Gz、± 8Gz暴露后的 6h和 2 4h组可见轻重不等的神经元变性。 3)电镜超微结构检查 :± 2Gz暴露后 30min和 2 4h组 ,海马CA1区神经元与正常对照组比较无明显改变。± 2Gz暴露后 6h组及± 6Gz、± 8Gz暴露后的 6h和 2 4h实验组可见大鼠海马CA1区部分神经元出现程度不等的变性。结论± 6Gz和± 8Gz暴露后可引起大鼠海马神经元损伤 ,且以± 8Gz暴露后 6h损伤程度最重。
[Abstract]:Objective to investigate the degree and temporal phase of rat hippocampal neuron injury induced by push-pull action of different intensities. Methods the injury of hippocampal neurons was measured by animal model score, pathology and electron microscopy. Results 1) Animal model score: with the increase of push-pull action intensity, stroke index and neurological symptom score increased gradually. 2) HE staining: the morphology and distribution of hippocampal neurons in control group, 卤2Gz group and 卤6Gz group and 卤8Gz group were not abnormal. 3) Ultrastructural examination of electron microscope: 30min and 24 h after 卤2Gz exposure; There was no significant change in hippocampal CA1 neurons compared with the normal control group. In the experimental group, there were different degrees of degeneration in the hippocampal CA1 area in the experimental group 6 hours after 卤2 Gz exposure and in 卤6 Gz and 卤8 Gz exposure at 6 h and 24 h after exposure. Conclusion 卤6 Gz and 卤8 Gz exposure can induce hippocampal neuron injury in rats, and the most serious injury is 6 h after 卤8 Gz exposure.
【作者单位】：第四军医大学吉林军医学院第四军医大学吉林军医学院第四军医大学吉林军医学院第四军医大学吉林军医学院第四军医大学吉林军医学院第四军医大学吉林军医学院
【基金】：全军医药科研基金项目 ( 0 1MA2 0 2 )
【分类号】：R85

【相似文献】