回转模拟失重对心肌成纤维细胞Ⅰ型胶原代谢的影响
发布时间：2018-07-13 13:21
【摘要】：为探讨模拟失重对心肌成纤维细胞Ⅰ型胶原代谢的影响 ,本研究采用回转器模拟失重效应 ,通过免疫细胞化学和反转录聚合酶链式反应 (RT PCR)研究了回转模拟失重对原代培养的新生大鼠心肌成纤维细胞Ⅰ型胶原蛋白及mRNA表达 ,以及胶原降解抑制物———金属蛋白酶组织抑制因子 (Tissueinhibitorofmetallopro teinase ,TIMP)mRNA表达的影响。免疫细胞化学染色显示回转组Ⅰ型胶原蛋白沉积增加 ;RT PCR分析显示Ⅰ型胶原α1链 (TypeⅠcollagenα1chain ,ColⅠA1)mRNA表达没有明显变化 ,TIMP 1、TIMP 2及TIMP 3的mRNA表达均增强。提示在回转模拟失重条件下 ,心肌成纤维细胞Ⅰ型胶原蛋白沉积增加 ,作为胶原降解抑制物的TIMP可能是造成胶原沉积的原因
[Abstract]:In order to investigate the effect of simulated weightlessness on collagen I metabolism in myocardial fibroblasts, a gyroscope was used to simulate the weightlessness. Immunocytochemistry and reverse transcription polymerase chain reaction (RT PCR) were used to study the expression of type I collagen and mRNA in primary cultured neonatal rat cardiac fibroblasts after rotational simulated weightlessness. And the effect of tissue inhibitor of metallopro teinase (TIMP) mRNA expression. Immunocytochemical staining showed that the type 鈪，
本文编号：2119512
[Abstract]:In order to investigate the effect of simulated weightlessness on collagen I metabolism in myocardial fibroblasts, a gyroscope was used to simulate the weightlessness. Immunocytochemistry and reverse transcription polymerase chain reaction (RT PCR) were used to study the expression of type I collagen and mRNA in primary cultured neonatal rat cardiac fibroblasts after rotational simulated weightlessness. And the effect of tissue inhibitor of metallopro teinase (TIMP) mRNA expression. Immunocytochemical staining showed that the type 鈪，
本文编号：2119512
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