离心机训练对大鼠不同组织mRNA表达的影响

发布时间：2018-07-23 17:39

【摘要】：目的分析 5个与离心机训练有关的基因在大鼠各组织的表达水平及其与训练时间的关系。方法分别提取离心机训练不同时间组大鼠心、脑、肾、肺和肠 5种组织的mRNA并进行量化 ,采用狭线杂交方法 ,用Dig 1 1 dUTP标记 5个用抑制性消减杂交筛选离心机训练大鼠获得的基因片段作探针 ,杂交结果通过光密度扫描半定量后 ,进行统计学处理。结果未知基因CH1 5 7的表达水平在训练 6d时大鼠心脏和脑组织明显下降 (P 0 .0 5 ) ,训练 1 2d时有所回升 ;未知基因CH2 4 4的表达水平在大鼠心、脑、肠组织在训练 6d、1 2d时均下调 ,但其表达量个体差异表现突出 ,不呈现明显的统计学意义 ;神经元一氧化氮合酶抑制剂 (PIN)基因 ,随着离心机训练时间的延长 ,在大鼠心脏和肾脏组织中的表达水平明显上调(P 0 .0 5 ) ;细胞色素b (Cytb)基因在大鼠心脏、脑组织中表达水平随着训练时间增长而明显上调 (P0 .0 1 ) ,在肾、肺和肠组织中的表达在训练 6d时明显升高 (P 0 .0 5 ) ,训练 1 2d时有所恢复 ;延长因子 1 α(EF1 α)基因在大鼠脑组织中表达水平随着训练时间增长而明显上调 ,在肠组织中的表达在训练 6d时明显升高 (P 0 .0 5 ) ,训练 1 2d时恢复到训练前水平。结论 5个基因在离心机训练大鼠不同组织中的表达水平存在差异 ,
[Abstract]:Objective to analyze the expression level of five genes related to centrifuge training in rat tissues and their relationship with training time. Methods the mRNA of heart, brain, kidney, lung and intestine of rats trained with centrifuge for different time groups were extracted and quantified. Dig 11 dUTP was used to label 5 gene fragments obtained from centrifuge training by suppression subtractive hybridization as probes. The hybridization results were processed by statistical analysis after semi-quantitative optical density scanning. Results the expression level of unknown gene CH1 57 was significantly decreased in the heart and brain of rats at 6 days of training (P 0. 05) and increased at 12 days, and the expression level of unknown gene CH2 44 was found in the heart and brain of rats. The intestinal tissue was down-regulated at 6 days and 12 days after training, but the expression level of intestinal tissue was significantly different from that of the control group, and there was no significant difference between them. The neuronal nitric oxide synthase inhibitor (PIN) gene increased with the training time of centrifuge. The expression of cytochrome b (Cytb) gene in rat heart and brain tissues increased significantly with the training time (P0. 01), and the expression level of cytochrome b (Cytb) gene in the rat heart and brain tissues increased significantly (P0. 01), and the expression of cytochrome b (Cytb) gene in the rat heart and brain tissue increased significantly (P0. 01) with the increase of training time (P0. 01). The expression of EF1 伪 in lung and intestine increased significantly at 6 days of training (P 0. 05) and recovered after 12 days of training, and the expression of EF1 伪 gene increased with the increase of training time. The expression level in intestinal tissue increased significantly at 6 days training (P 0. 05), and recovered to pre training level at 12 days training. Conclusion there are differences in the expression levels of five genes in different tissues of centrifuge training rats.
【作者单位】：中山大学中山医学院生化教研室空军总医院空军临床分子生物学中心实验室空军航空医学研究所中山大学中山医学院生化教研室
【基金】：全军“十五”重点课题 ( 0 1Z0 16) 空军后勤部“十五”规划项目 (KH0 0 190 93 )
【分类号】：R85

【相似文献】