军事噪声性听力损失的线粒体基因变异分析

发布时间：2018-07-31 08:55

【摘要】：目的研究线粒体基因变异与军事噪声性听力损失遗传易感性的关系,为确定噪声易感个体的分子诊断方法提供依据。方法对云南某部接触军事噪声的406名坦克兵、626名炮兵共计1032例进行听力损失调查,收集军事噪声易感者(易感组)82例,耐受者(耐受组)40例,共计122例。分别采集外周静脉血标本,从白细胞中提取DNA,PCR扩增线粒体DNA及非综合征型耳聋相关的热点突变部分片段,进行基因测序比对分析,比较两组中存在碱基改变的位点分布例数。对测序结果中存在与耳聋相关的碱基改变的个体做进一步的听力学分析。结果军事噪声易感者和耐受者的线粒体基因存在一定差异,线粒体COII基因T7684C和G7853A两个位点的变化只存在于易感组,两组间有显著性差异(P0.05)。A827G、T961insC(异质)、T1005C、T1095C、G7444A等可能与耳聋相关的线粒体基因改变多见于易感组,耐受组也有分布。结论携带线粒体COII基因T7684C和G7853A碱基变化的个体可能对军事噪声更加易感。
[Abstract]:Objective to study the relationship between mitochondrial gene variation and the genetic susceptibility to military noise-induced hearing loss in order to provide a basis for the molecular diagnosis of noise-susceptible individuals. Methods A total of 1032 artillery soldiers exposed to military noise were investigated for hearing loss. 82 cases were susceptible to military noise (susceptible group), 40 cases were tolerant group (tolerance group), a total of 122 cases. The peripheral venous blood samples were collected and the mitochondrial DNA and non-syndromic deafness related hotspots were amplified by DNA polymerase chain reaction (DNA-PCR) from white blood cells. The gene sequencing was carried out to compare the number of loci with base changes in the two groups. Further audiological analysis was performed on individuals with base changes associated with deafness as a result of sequencing. Results there were some differences in mitochondrial gene between susceptible and tolerant subjects. The changes of T7684C and G7853A loci of mitochondrial COII gene only existed in susceptible group. There was a significant difference between the two groups (P0.05). A827GN T961insC (heterogeneity) T1005CnT1095CnG7444A may be related to deafness in the susceptible group, and the tolerance group was also distributed. Conclusion individuals carrying mitochondrial COII gene T7684C and G7853A base changes may be more susceptible to military noise.
【作者单位】：
【基金】：全军医药卫生“十一五”科技攻关项目(06G110) 全军医药卫生“十一五”科研基金面上项目(06MA142)
【分类号】：R82

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